Human GAP43 Recombinant Protein (RPPB3579)

(No reviews yet) Write a Review
SKU:
RPPB3579
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17677

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human GAP43 Recombinant Protein
Product Code:RPPB3579
Size:10µg
Species:Human
Target:GAP43
Synonyms:Growth associated protein 43, Axonal membrane protein GAP-43, Neural phosphoprotein B-50, calmodulin-binding protein P-57, nerve growth-related peptide GAP43, neuromodulin, protein F1, pp46, B-50.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GAP43 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMLCCMR RTKQVEKNDD DQKIEQDGIK PEDKAHKAAT KIQASFRGHI TRKKLKGEKK DDVQAAEAEA NKKDEAPVAD GVEKKGEGTT TAEAAPATGS KPDEPGKAGE TPSEEKKGEG DAATEQAAPQ APASSEEKAG SAETESATKA STDNSPSSKA EDAPAKEEPK QADVPAAVTA AAATTPAAED AAAKATAQPP TETGESSQAE ENIEAVDETK PKESARQDEG KEEEPEADQE HA

GAP43 is a phosphoprotein of the presynaptic membrane that takes part in synaptic reorganization and neural development. GAP43 is found in large quantities in neuronal growth cones all through the development and axonal regeneration. Additionally, GAP43 is involved in specific regions of the normal adult nervous system and has a vital part in the conservation mechanism of long-term potentiation (LTP).

GAP43 Human Recombinant produced in E. coli is a single polypeptide chain containing 262 amino acids (1-238) and having a molecular mass of 27.3kDa (Molecular size on SDS-PAGE will appear higher).GAP43 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: axon; cell junction; cytoplasm; filopodium membrane; plasma membrane; postsynaptic density

Molecular Function:calmodulin binding; phosphatidylserine binding; protein binding

Biological Process: axon choice point recognition; cell fate commitment; glial cell differentiation; protein kinase C activation; regulation of filopodium formation; regulation of growth; response to wounding; tissue regeneration

NCBI Summary:The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P17677
NCBI GenInfo Identifier:128100
NCBI Gene ID:2596
NCBI Accession:P17677.1
UniProt Secondary Accession:P17677,A8K0Y4,
UniProt Related Accession:P17677
Molecular Weight:
NCBI Full Name:Neuromodulin
NCBI Synonym Full Names:growth associated protein 43
NCBI Official Symbol:GAP43��
NCBI Official Synonym Symbols:B-50; PP46��
NCBI Protein Information:neuromodulin
UniProt Protein Name:Neuromodulin
UniProt Synonym Protein Names:Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46
Protein Family:Neuromodulin
UniProt Gene Name:GAP43��
UniProt Entry Name:NEUM_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products