Enzymes Recombinant Proteins

Human GAD2 Recombinant Protein (RPPB1665)

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SKU:
RPPB1665
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q05329
Research Area:
Enzymes

Description

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Product Name:Human GAD2 Recombinant Protein
Product Code:RPPB1665
Size:100µg
Species:Human
Target:GAD2
Synonyms:Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa), Glutamate Decarboxylase 65 KDa Isoform, 65 KDa Glutamic Acid Decarboxylase, EC 4.1.1.15, GAD-65, GAD65, Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD), Glutamate Decarboxylase-2 (Pancreas), EC 4.1.1, GAD2.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:GAD2 protein solution is supplied in 16mM HEPES buffer pH-7.5, 250mM NaCl, 0.016mM Pyridoxal-5�-Phosphate, 0.16% Triton x-100 and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.

Glutamate Decarboxylase 2 (GAD2) is one of several forms of glutamic acid decarboxylase, identified as a main autoantigen in�type II diabetes. The GAD2 enzyme is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for the GAD2 enzyme has been characterized in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in�type II diabetes. GAD2 gene may also have a role in the stiff man syndrome. In addition, GAD2 catalyzes the production of GABA.

Recombinant Human GAD2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66,234 Dalton. GAD2 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - taurine and hypotaurine; Lyase; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

NCBI Summary:This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
UniProt Code:Q05329
NCBI GenInfo Identifier:1352216
NCBI Gene ID:2572
NCBI Accession:Q05329.1
UniProt Related Accession:Q05329
Molecular Weight:
NCBI Full Name:Glutamate decarboxylase 2
NCBI Synonym Full Names:glutamate decarboxylase 2
NCBI Official Symbol:GAD2��
NCBI Official Synonym Symbols:GAD65��
NCBI Protein Information:glutamate decarboxylase 2
UniProt Protein Name:Glutamate decarboxylase 2
UniProt Synonym Protein Names:65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Protein Family:Glutamate decarboxylase
UniProt Gene Name:GAD2��
UniProt Entry Name:DCE2_HUMAN
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