Enzymes Recombinant Proteins

Human GAD1 iso1 Recombinant Protein (RPPB1664)

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SKU:
RPPB1664
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99259
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human GAD1 iso1 Recombinant Protein
Product Code:RPPB1664
Size:20µg
Species:Human
Target:GAD1 iso1
Synonyms:Glutamate Decarboxylase 1 (Brain, 67kDa), GlutamateDecarboxylase 67 KDa Isoform, 67 KDa Glutamic Acid Decarboxylase, EC 4.1.1.15,GAD-67, CPSQ1, SCP, GAD, Glutamate Decarboxylase 1 (Brain, 67kD), GlutamateDecarboxylase 1, EC 4.1.1, GAD67, Glutamate decarboxylase 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GAD1 iso1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKICGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFLLEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLELSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTSTANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMAAVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYVPFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHRHKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGRHVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWYIPQSLRGVPD SPQRREKLHKVAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL

Glutamate Decarboxylase 1 Isoform-1, also known as GAD1 iso1 belongs tothe group II decarboxylase family. GAD1 iso1 encodes one of several forms ofglutamic acid decarboxylase, and is identified as a major autoantigen ininsulin-dependent diabetes. GAD1 iso1 is in charge for catalyzing theproduction of gamma-aminobutyric acid from L-glutamic acid. In addition, apathogenic function for GAD1 iso1 has been shown in the human pancreas while ithas been identified as an autoantigen & an autoreactive T cell target ininsulin-dependent diabetes. GAD1 iso1 play a role in the stiff man syndrome. Ithas been shown that deficiency in this enzyme has led to pyridoxine dependencywith seizures.

GAD1 iso1 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 617 amino acids (1-594 a.a) andhaving a molecular mass of 69.3kDa.GAD1 iso1 is fused to a 23 amino acid His-tag atN-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; intracellular; vesicle membrane

Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1

NCBI Summary:This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
UniProt Code:Q99259
NCBI GenInfo Identifier:1352213
NCBI Gene ID:2571
NCBI Accession:Q99259.1
UniProt Secondary Accession:Q99259,Q49AK1, Q53TQ7, Q9BU91, Q9UHH4,
UniProt Related Accession:Q99259
Molecular Weight:66,897 Da
NCBI Full Name:Glutamate decarboxylase 1
NCBI Synonym Full Names:glutamate decarboxylase 1 (brain, 67kDa)
NCBI Official Symbol:GAD1��
NCBI Official Synonym Symbols:GAD; SCP; CPSQ1��
NCBI Protein Information:glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform
UniProt Protein Name:Glutamate decarboxylase 1
UniProt Synonym Protein Names:67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family:Glutamate decarboxylase
UniProt Gene Name:GAD1��
UniProt Entry Name:DCE1_HUMAN
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