Human GABARAPL2 Recombinant Protein (RPPB3569)

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SKU:
RPPB3569
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P60520

Description

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Product Name:Human GABARAPL2 Recombinant Protein
Product Code:RPPB3569
Size:10µg
Species:Human
Target:GABARAPL2
Synonyms:GABA(A) receptor-associated protein-like 2, GATE-16, GEF2, ATG8, ATG8C, Ganglioside expression factor 2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, MAP1 light chain 3-related protein, gamma-aminobutyric acid receptor-associated protein-like 2, MAP1 light chain 3 related protein, FLC3A.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The GABARAPL2 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

GABARAPL2 takes part in intra Golgi traffic and modulates intra Golgi transport by coupling between NSF activity and SNAREs activation. GABARAPL2 first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

GABARAPL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 137 amino acids (1-117a.a.) and having a molecular mass of 15.8kDa. GABARAPL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR.
UniProt Protein Details:

Protein type:Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular

Molecular Function:ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity

UniProt Code:P60520
NCBI GenInfo Identifier:44888808
NCBI Gene ID:11345
NCBI Accession:P60520.1
UniProt Secondary Accession:P60520,O08765, Q6FG91, Q9DCP8, Q9UQF7,
UniProt Related Accession:P60520
Molecular Weight:13,667 Da
NCBI Full Name:Gamma-aminobutyric acid receptor-associated protein-like 2
NCBI Synonym Full Names:GABA type A receptor associated protein like 2
NCBI Official Symbol:GABARAPL2��
NCBI Official Synonym Symbols:ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16��
NCBI Protein Information:gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Protein Name:Gamma-aminobutyric acid receptor-associated protein-like 2
UniProt Synonym Protein Names:GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein
Protein Family:Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:GABARAPL2��
UniProt Entry Name:GBRL2_HUMAN
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