Human GABARAPL1 Recombinant Protein (RPPB3568)

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RPPB3568

Description

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Product Name:Human GABARAPL1 Recombinant Protein
Product Code:RPPB3568
Size:20µg
Species:Human
Target:GABARAPL1
Synonyms:Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1, GABARAPL1, GEC1, ATG8, APG8L, ATG8B, ATG8L, APG8-LIKE.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GABARAPL1 solution (1 mg/ml) contains 20mM Tris buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE EDYFLYVAYS DESVYGK

GABARAPL1 [Gamma-aminobutyric acid (GABA) receptor-associated protein-like 1] is a member of the MAP1 LC3 family. GABARAPL1 increases cell surface expression of kappa type opioid receptor through facilitating anterograde intracellular trafficking of the receptor.

GABARAPL1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16.2kDa (Molecular size on SDS-PAGE will appear higher). The GABARAPL1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1.
UniProt Protein Details:

Protein type:Vesicle; Adaptor/scaffold; Microtubule-binding; Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: autophagic vacuole; cytoplasmic vesicle membrane; cytosol; dendrite cytoplasm; endoplasmic reticulum; Golgi apparatus; intracellular; microtubule; mitochondrion

Molecular Function:beta-tubulin binding; GABA receptor binding; protein binding; Tat protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; macroautophagy; mitochondrion degradation

UniProt Code:Q9H0R8
NCBI GenInfo Identifier:13899219
NCBI Gene ID:23710
NCBI Accession:NP_113600.1
UniProt Secondary Accession:Q9H0R8,Q6FIE6, B4E0Y7,
UniProt Related Accession:Q9H0R8
Molecular Weight:17,008 Da
NCBI Full Name:gamma-aminobutyric acid receptor-associated protein-like 1
NCBI Synonym Full Names:GABA type A receptor associated protein like 1
NCBI Official Symbol:GABARAPL1��
NCBI Official Synonym Symbols:ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE��
NCBI Protein Information:gamma-aminobutyric acid receptor-associated protein-like 1
UniProt Protein Name:Gamma-aminobutyric acid receptor-associated protein-like 1
UniProt Synonym Protein Names:Early estrogen-regulated protein; GABA(A) receptor-associated protein-like 1; Glandular epithelial cell protein 1; GEC-1
Protein Family:Gamma-aminobutyric acid receptor-associated protein
UniProt Gene Name:GABARAPL1��
UniProt Entry Name:GBRL1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H0R8
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system_update_altDatasheetHuman GABARAPL1 Recombinant Protein Gamma-Aminobutyric Acid Receptor-Associated Protein-Like 1 (GABARAPL1) is a cytoplasmic protein that belongs to the MAP1 LC3 family.