Human FDX1 Recombinant Protein (RPPB3532)

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SKU:
RPPB3532
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P10109

Description

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Product Name:Human FDX1 Recombinant Protein
Product Code:RPPB3532
Size:25µg
Species:Human
Target:FDX1
Synonyms:Adrenodoxin mitochondrial, Adrenal ferredoxin, Ferredoxin-1, Hepatoredoxin, FDX1, ADX.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The FDX1 solution contains 20mM Tris-HCl buffer (pH8.0) and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS

Ferredoxin-1 is a small iron-sulfur protein who transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This specific oxidation/reduction system is located in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. FDX1 takes part in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. FDX1 supports reactions catalyzed by human microsomal P450s- full length CYP17, truncated CYP17, and truncated CYP21. In addition to the FDX1 at the 11q22chromosomal locus, there are pseudogenes located on chromosomes 20 and 21.

FDX1 Human Recombinant fused with a 15 amino acid T7 tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 139 amino acids (61-184 a.a.) and having a molecular mass of 15.0kDa.The FDX1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:adrenodoxin: Participates in the synthesis of thyroid hormones. Essential for the synthesis of various steroid hormones, participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to cytochrome P450 cholesterol side-chain cleavage enzyme. Belongs to the adrenodoxin/putidaredoxin family.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 11q22

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:2 iron, 2 sulfur cluster binding; electron carrier activity; iron ion binding

Biological Process: steroid metabolic process; cholesterol metabolic process; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; hormone biosynthetic process; sterol metabolic process

NCBI Summary:This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. [provided by RefSeq, Aug 2011]
UniProt Code:P10109
NCBI GenInfo Identifier:4758352
NCBI Gene ID:2230
NCBI Accession:NP_004100.1
UniProt Secondary Accession:P10109,Q53YD6, B0YJ14,
UniProt Related Accession:P10109
Molecular Weight:19,393 Da
NCBI Full Name:adrenodoxin, mitochondrial
NCBI Synonym Full Names:ferredoxin 1
NCBI Official Symbol:FDX1��
NCBI Official Synonym Symbols:ADX; FDX; LOH11CR1D��
NCBI Protein Information:adrenodoxin, mitochondrial; ferredoxin-1; hepatoredoxin; adrenal ferredoxin; mitochondrial adrenodoxin
UniProt Protein Name:Adrenodoxin, mitochondrial
UniProt Synonym Protein Names:Adrenal ferredoxin; Ferredoxin-1; Hepatoredoxin
Protein Family:Adrenodoxin
UniProt Gene Name:FDX1��
UniProt Entry Name:ADX_HUMAN
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