Enzymes Recombinant Proteins

Human FBP2 Recombinant Protein (RPPB1635)

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SKU:
RPPB1635
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00757
Research Area:
Enzymes

Description

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Product Name:Human FBP2 Recombinant Protein
Product Code:RPPB1635
Size:20µg
Species:Human
Target:FBP2
Synonyms:Fructose-1,6-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2, FBPase 2, D-fructose-1,6-bisphosphate 1-phosphohydrolase 2, FBP2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The FBP2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMTDRSPF ETDMLTLTRY VMEKGRQAKG TGELTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS

Fructose-1,6-bisphosphatase isozyme 2 (FBP2) is a part of the FBPase class 1 family. FBP2 is a gluconeogenesis regulatory enzyme Which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate.

FBP2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 362 amino acids (1-339) and having a molecular mass of 39kDa. FBP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.
UniProt Protein Details:

Protein type:EC 3.1.3.11; Carbohydrate Metabolism - pentose phosphate pathway; Phosphatase (non-protein); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytosol

Molecular Function:fructose-bisphosphatase activity; protein binding

Biological Process: fructose metabolic process; gluconeogenesis

NCBI Summary:This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]
UniProt Code:O00757
NCBI GenInfo Identifier:76789651
NCBI Gene ID:8789
NCBI Accession:O00757.2
UniProt Secondary Accession:O00757,Q17R39, Q6FI53,
UniProt Related Accession:O00757
Molecular Weight:36,743 Da
NCBI Full Name:Fructose-1,6-bisphosphatase isozyme 2
NCBI Synonym Full Names:fructose-bisphosphatase 2
NCBI Official Symbol:FBP2��
NCBI Protein Information:fructose-1,6-bisphosphatase isozyme 2
UniProt Protein Name:Fructose-1,6-bisphosphatase isozyme 2
UniProt Synonym Protein Names:D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; Muscle FBPase
Protein Family:Fat body protein
UniProt Gene Name:FBP2��
UniProt Entry Name:F16P2_HUMAN
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