Enzymes Recombinant Proteins
Human FBP1 Recombinant Protein (RPPB1634)
- SKU:
- RPPB1634
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09467
- Research Area:
- Enzymes
Description
| Product Name: | Human FBP1 Recombinant Protein |
| Product Code: | RPPB1634 |
| Size: | 10µg |
| Species: | Human |
| Target: | FBP1 |
| Synonyms: | Fructose-1,6-bisphosphatase 1, FBPase 1, �D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, Liver FBPase, FBP1, FBP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | FBP1 protein solution (1mg/ml) contains 1mM DTT, 10% glycerol and 20mM Tris-HCl buffer (pH 8.0). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ |
| Biological Activity: | Specific activity is > 7,000pmol/min/ug, and is determined by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. 1 unit oxidizes 1.0pmole of fructose 1,6 diphosphate to fructose 6- phosphate and inorganic phosphate per minute at pH 9.5 at 37?C. |
FBP1 or Fructose-1, 6-bisphosphatase 1 is an enzyme, catalyzing the formation of fructose 6-phosphate & inorganic phosphate from fructose 1, 6-bisphosphate. FBP1 is part of the gluconeogenesis regulatory enzymes. Mutations in the enzyme gene can result in metabolic acidosis & hypoglycemia.
FBP1 Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 358 amino acids (1-338) and having a molecular mass of 39.0 kDa.FBP1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FBPase: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans, FBP1 in the liver and FBP2 in muscle. While both forms are inhibited allosterically by AMP, NAD and Ca2+, the muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Deficiency of FBP1 leads to a disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children. |
| UniProt Protein Details: | Protein type:Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; EC 3.1.3.11; Carbohydrate Metabolism - fructose and mannose; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis Chromosomal Location of Human Ortholog: 9q22.3 Cellular Component: cytoplasm; cytosol Molecular Function:monosaccharide binding; identical protein binding; protein binding; metal ion binding; fructose-bisphosphatase activity; AMP binding Biological Process: dephosphorylation; carbohydrate metabolic process; negative regulation of Ras protein signal transduction; glucose metabolic process; regulation of gluconeogenesis; pathogenesis; negative regulation of cell growth; negative regulation of glycolysis; fructose 6-phosphate metabolic process; protein homotetramerization; fructose metabolic process; gluconeogenesis Disease: Fructose-1,6-bisphosphatase Deficiency |
| NCBI Summary: | Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09467 |
| NCBI GenInfo Identifier: | 311033495 |
| NCBI Gene ID: | 2203 |
| NCBI Accession: | P09467.5 |
| UniProt Secondary Accession: | P09467,O75571, Q53F94, Q96E46, |
| UniProt Related Accession: | P09467 |
| Molecular Weight: | 338 |
| NCBI Full Name: | Fructose-1,6-bisphosphatase 1 |
| NCBI Synonym Full Names: | fructose-1,6-bisphosphatase 1 |
| NCBI Official Symbol: | FBP1�� |
| NCBI Official Synonym Symbols: | FBP�� |
| NCBI Protein Information: | fructose-1,6-bisphosphatase 1; FBPase 1; liver FBPase; fructose-bisphosphatase 1; growth-inhibiting protein 17; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1 |
| UniProt Protein Name: | Fructose-1,6-bisphosphatase 1 |
| UniProt Synonym Protein Names: | D-fructose-1,6-bisphosphate 1-phosphohydrolase 1; Liver FBPase |
| Protein Family: | Fat-body protein |
| UniProt Gene Name: | FBP1�� |
| UniProt Entry Name: | F16P1_HUMAN |
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