Enzymes Recombinant Proteins

Human FARSB Recombinant Protein (RPPB1631)

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RPPB1631

Description

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Product Name:Human FARSB Recombinant Protein
Product Code:RPPB1631
Size:20µg
Species:Human
Target:FARSB
Synonyms:Phenylalanyl-TRNASynthetase, Beta Subunit, FARSLB,FRSB, Phenylalanyl-TRNA Synthetase-Like, BetaSubunit, EC 6.1.1.20, PheRS, Phenylalanine-TRNA Synthetase-Like, Beta Subunit,Phenylalanine TRNA Ligase 1, Beta, Cytoplasmic, Phenylalanyl-TRNA SynthetaseBeta-Subunit, Phenylalanyl-TRNA Synthetase Beta Subunit, Phenylalanine--TRNALigase Beta Subunit, Phenylalanyl-TRNA Synthetase Beta Chain,Phenylalanine--TRNA Ligase Beta Chain, Phenylalanine-TRNA Ligase Beta Chain,Phenylalanine TRNA Ligase 1, Cytoplasmic, EC 6.1.1, HSPC173,PheHB,Beta,FARSB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:FARSB protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMPTVSVK RDLLFQALGR TYTDEEFDEL CFEFGLELDE ITSEKEIISK EQGNVKAAGA SDVVLYKIDV PANRYDLLCL EGLVRGLQVF KERIKAPVYK RVMPDGKIQK LIITEETAKI RPFAVAAVLR NIKFTKDRYD SFIELQEKLH QNICRKRALV AIGTHDLDTL SGPFTYTAKR PSDIKFKPLN KTKEYTACEL MNIYKTDNHL KHYLHIIENK PLYPVIYDSN GVVLSMPPII NGDHSRITVN TRNIFIECTG TDFTKAKIVL DIIVTMFSEY CENQFTVEAA EVVFPNGKSH TFPELAYRKE MVRADLINKK VGIRETPENL AKLLTRMYLK SEVIGDGNQI EIEIPPTRAD IIHACDIVED AAIAYGYNNI QMTLPKTYTI ANQFPLNKLT ELLRHDMAAA GFTEALTFAL CSQEDIADKL GVDISATKAV HISNPKTAEF QVARTTLLPG LLKTIAANRK MPLPLKLFEI SDIVIKDSNT DVGAKNYRHL CAVYYNKNPG FEIIHGLLDR IMQLLDVPPG EDKGGYVIKA SEGPAFFPGR CAEIFARGQS VGKLGVLHPD VITKFELTMP CSSLEINVGP FL

FARSB, also known as Phenylalanyl-TRNASynthetase Beta is a member of the phenylalanyl-tRNA synthetase betasubunit family. FARSB is composed tetramer of two alpha and two beta subunits.In the presence of ATP, this tetramer is accountable for attaching L-phenylalanineto the terminal adenosine of the appropriate tRNA.

FARSB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 612 amino acids (1-589 a.a) and having a molecular mass of 68.5kDa.FARSB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:FARSLB: a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Ligase; EC 6.1.1.20; Translation

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:RNA binding; phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; phenylalanyl-tRNA aminoacylation; gene expression

NCBI Summary:This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:Q9NSD9
NCBI GenInfo Identifier:296452943
NCBI Gene ID:10056
NCBI Accession:Q9NSD9.3
UniProt Related Accession:Q9NSD9
Molecular Weight:
NCBI Full Name:Phenylalanine--tRNA ligase beta subunit
NCBI Synonym Full Names:phenylalanyl-tRNA synthetase subunit beta
NCBI Official Symbol:FARSB��
NCBI Official Synonym Symbols:FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA��
NCBI Protein Information:phenylalanine--tRNA ligase beta subunit
UniProt Protein Name:Phenylalanine--tRNA ligase beta subunit
UniProt Synonym Protein Names:Phenylalanyl-tRNA synthetase beta subunit; PheRS
UniProt Gene Name:FARSB��
UniProt Entry Name:SYFB_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NSD9
Research Area:
Enzymes
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