Human F1+2 / Prothrombin Fragment 1+2 ELISA Kit
- SKU:
- HUFI02435
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P00734
- Sensitivity:
- 28.125pg/ml
- Range:
- 46.875-3000pg/ml
- ELISA Type:
- Sandwich
- Synonyms:
- F1+2
- Reactivity:
- Human
Description
| Product Name: | Human F1+2 / Prothrombin Fragment 1+2 ELISA Kit |
| Product Code: | HUFI02435 |
| Size: | 96 Assays |
| Alias: | F1+2 |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human F1+2 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 28.125pg/ml |
| Range: | 46.875-3000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human F1+2 in samples. |
| |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
| |
| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P00734 |
| UniProt Protein Function: | Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Ref.12 |
| UniProt Protein Details: | Catalytic activity: Selective cleavage of Arg--Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B. Subcellular location: Secreted º extracellular space. Tissue specificity: Expressed by the liver Fill out our quote form below and a dedicated member of staff will get back to you within one working day! |
