Human EXOSC8 Recombinant Protein (RPPB3478)

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RPPB3478

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Product Name:Human EXOSC8 Recombinant Protein
Product Code:RPPB3478
Size:10µg
Species:Human
Target:EXOSC8
Synonyms:Exosome Component 8, EXOSC8, OIP2, RRP43, CBP-Interacting Protein 3, Opa Interacting Protein 2, Opa-Interacting Protein 2, Ribosomal RNA-Processing Protein 43, OIP-2, p9, CIP3, EAP2, Rrp43p, bA421P11.3, Exosome Complex Component RRP43, Exosome Complex Exonuclease RRP43.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The EXOSC8 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.2M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAGFKT VEPLEYYRRF LKENCRPDGR ELGEFRTTTV NIGSISTADG SALVKLGNTT VICGVKAEFA APSTDAPDKG YVVPNVDLPP LCSSRFRSGP PGEEAQVASQ FIADVIENSQ IIQKEDLCIS PGKLVWVLYC DLICLDYDGN ILDACTFALL AALKNVQLPE VTINEETALA EVNLKKKSYL NIRTHPVATS FAVFDDTLLI VDPTGEEEHL ATGTLTIVMD EEGKLCCLHK PGGSGLTGAK LQDCMSRAVT RHKEVKKLMD EVIKSMKPK

Exosome component 8 and transcription factor 4 (EXOSC8) is a part of the exosome complex. In the cytoplasm, the RNA exosome complex is engaged in general mRNA turnover and specifically degrades naturally unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA inspection pathways, preventing translation of aberrant mRNAs. EXOSC8 appears to be involved in degradation of histone mRNA. EXOSC8 attaches to ARE-containing RNAs. The EXOSC8 gene encodes a 3'-5' exoribonuclease, which specifically interacts with mRNAs containing AU-rich elements. The EXOSC8 protein is part of the exosome complex, which is significant for the degradation of many RNA species.

EXOSC8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276) and having a molecular mass of 32.4 kDa.EXOSC8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EXOSC8: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs. Belongs to the RNase PH family.
UniProt Protein Details:

Protein type:Nucleolus; EC 3.1.13.-; Ribonuclease

Chromosomal Location of Human Ortholog: 13q13.1

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); nucleus; cytosol

Molecular Function:protein binding; exoribonuclease activity; AU-rich element binding

Biological Process: gene expression; rRNA processing; mRNA catabolic process, deadenylation-dependent decay

Disease: Pontocerebellar Hypoplasia, Type 1c

NCBI Summary:This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2009]
UniProt Code:Q96B26
NCBI GenInfo Identifier:21759409
NCBI Gene ID:11340
NCBI Accession:Q96B26.1
UniProt Secondary Accession:Q96B26,O43480, Q5TBA5,
UniProt Related Accession:Q96B26
Molecular Weight:276
NCBI Full Name:Exosome complex component RRP43
NCBI Synonym Full Names:exosome component 8
NCBI Official Symbol:EXOSC8��
NCBI Official Synonym Symbols:p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3��
NCBI Protein Information:exosome complex component RRP43; OIP-2; CBP-interacting protein 3; Opa interacting protein 2; opa-interacting protein 2; exosome complex exonuclease RRP43; ribosomal RNA-processing protein 43
UniProt Protein Name:Exosome complex component RRP43
UniProt Synonym Protein Names:Exosome component 8; Opa-interacting protein 2; OIP-2; Ribosomal RNA-processing protein 43; p9
Protein Family:Exosome complex component
UniProt Gene Name:EXOSC8��
UniProt Entry Name:EXOS8_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96B26
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