Human EXOSC7 Recombinant Protein (RPPB3477)

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SKU:
RPPB3477
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15024

Description

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Product Name:Human EXOSC7 Recombinant Protein
Product Code:RPPB3477
Size:20µg
Species:Human
Target:EXOSC7
Synonyms:Exosome Component 7, RRP42, Ribosomal RNA-Processing Protein 42, P8, Exosome Complex Exonuclease RRP42, Exosome Complex Component RRP42, KIAA0116, HRrp42p, EAP1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EXOSC7 protein solution (0.5mg/ml) containing 20mM Phosphate buffer saline (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASVTLS EAEKVYIVHG VQEDLRVDGR GCEDYRCVEV ETDVVSNTSG SARVKLGHTD ILVGVKAEMG TPKLEKPNEG YLEFFVDCSA SATPEFEGRG GDDLGTEIAN TLYRIFNNKS SVDLKTLCIS PREHCWVLYV DVLLLECGGN LFDAISIAVK AALFNTRIPR VRVLEDEEGS KDIELSDDPY DCIRLSVENV PCIVTLCKIG YRHVVDATLQ EEACSLASLL VSVTSKGVVT CMRKVGKGSL DPESIFEMME TGKRVGKVLH ASLQSVVHKE ESLGPKRQKV GFLG�

EXOSC7, a component of the RNA exosome complex, is a member of the RNase PH family. EXOSC7 takes part is several pathways such as transfer to a nascent protein and Unfolded Protein Response and Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO). A significant paralog of this gene is EXOSC9.

EXOSC7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-291 a.a.) and having a molecular mass of 34.2kDa.EXOSC7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EXOSC7: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Belongs to the RNase PH family.
UniProt Protein Details:

Protein type:EC 3.1.13.-; Nucleolus; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleoplasm; nucleus

Molecular Function:3'-5'-exoribonuclease activity; AU-rich element binding; exoribonuclease activity; protein binding; RNA binding

Biological Process: exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); regulation of mRNA stability; rRNA processing

UniProt Code:Q15024
NCBI GenInfo Identifier:189083688
NCBI Gene ID:23016
NCBI Accession:NP_055819
UniProt Secondary Accession:Q15024,Q96E72,
UniProt Related Accession:Q15024
Molecular Weight:34.2 kDa (314aa) confirmed by MALDI-TOF
NCBI Full Name:exosome complex component RRP42
NCBI Synonym Full Names:exosome component 7
NCBI Official Symbol:EXOSC7��
NCBI Official Synonym Symbols:p8; EAP1; RRP42; Rrp42p; hRrp42p��
NCBI Protein Information:exosome complex component RRP42
UniProt Protein Name:Exosome complex component RRP42
UniProt Synonym Protein Names:Exosome component 7; Ribosomal RNA-processing protein 42; p8
Protein Family:Exosome complex exonuclease
UniProt Gene Name:EXOSC7��
UniProt Entry Name:EXOS7_HUMAN
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