Human EXOSC5 Recombinant Protein (RPPB3476)

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SKU:
RPPB3476
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NQT4

Description

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Product Name:Human EXOSC5 Recombinant Protein
Product Code:RPPB3476
Size:25µg
Species:Human
Target:EXOSC5
Synonyms:Exosome component 5, Chronic myelogenous leukemia tumor antigen 28, Ribosomal RNA-processing protein 46, exosome complex exonuclease RRP46, RRP41B, hRrp46p, CML28, p12B.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The EXOSC5 solution (0.5mg/1ml) contains PBS buffer (pH 7.4), 200mM Nacl, 2mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MMEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS

EXOSC5 is a member of the RNase PH family. EXOSC5 is known to cooperate with Exosome component 8 and Exosome component 1. EXOSC5 is a Non-catalytic component of the RNA exosome complex and has 3'->5' exoribonuclease activity. EXOSC5 take part in a large number of cellular RNA processing and degradation events. EXOSC5 is expressed in a large range of hematopoietic and epithelial tumor cell lines, but not in standard hematopoietic tissues or other standard tissue, with the exception of testis.

EXOSC5 Human Recombinant produced in�E. coli is a single polypeptide chain containing 256 amino acids (1-235) and having a molecular mass of 27.5kDa.EXOSC5 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EXOSC5: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Belongs to the RNase PH family.
UniProt Protein Details:

Protein type:Ribonuclease; Nucleolus; EC 3.1.13.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; nucleolus; exosome (RNase complex); nucleus; cytosol

Molecular Function:protein binding; 3'-5'-exoribonuclease activity; exoribonuclease activity; RNA binding

Biological Process: gene expression; DNA deamination; defense response to virus; rRNA processing; mRNA catabolic process, deadenylation-dependent decay

UniProt Code:Q9NQT4
NCBI GenInfo Identifier:14285757
NCBI Gene ID:56915
NCBI Accession:Q9NQT4.1
UniProt Related Accession:Q9NQT4
Molecular Weight:Calculated: 25kDaObserved: 25kDa
NCBI Full Name:Exosome complex component RRP46
NCBI Synonym Full Names:exosome component 5
NCBI Official Symbol:EXOSC5��
NCBI Official Synonym Symbols:p12B; RRP46; RRP41B; Rrp46p; hRrp46p��
NCBI Protein Information:exosome complex component RRP46
UniProt Protein Name:Exosome complex component RRP46
UniProt Synonym Protein Names:Chronic myelogenous leukemia tumor antigen 28; Exosome component 5; Ribosomal RNA-processing protein 46; p12B
Protein Family:Exosome complex component
UniProt Gene Name:EXOSC5��
UniProt Entry Name:EXOS5_HUMAN
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