Human EXOSC4 Recombinant Protein (RPPB3475)

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RPPB3475

Description

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Product Name:Human EXOSC4 Recombinant Protein
Product Code:RPPB3475
Size:20µg
Species:Human
Target:EXOSC4
Synonyms:Exosome Component 4, Ribosomal RNA-Processing Protein 41, Exosome Complex Exonuclease RRP41, Exosome Complex Component RRP41, RRP41A, Ski6p, SKI6, p12A.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:EXOSC4 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGLELL SDQGYRVDGR RAGELRKIQA RMGVFAQADG SAYIEQGNTK ALAVVYGPHE IRGSRARALP DRALVNCQYS SATFSTGERK RRPHGDRKSC EMGLQLRQTF EAAILTQLHP RSQIDIYVQV LQADGGTYAA CVNAATLAVL DAGIPMRDFV CACSAGFVDG TALADLSHVE EAAGGPQLAL ALLPASGQIA LLEMDARLHE DHLERVLEAA AQAARDVHTL LDRVVRQHVR EASILLGD�

EXOSC4 is a Non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and takes part in several cellular RNA processing and degradation procedures. In the nucleus, the RNA exosome complex contributes to accurate maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the removal of RNA processing by-products and non-coding 'pervasive' transcripts, like antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing deficiencies, thus controlling or excluding their export to the cytoplasm.�Diseases related with EXOSC4 include hepatitis a, and mouth disease.

EXOSC4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 268 amino acids (1-245aa) and having a molecular mass of 28.8kDa.EXOSC4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EXOSC4: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs. Belongs to the RNase PH family.
UniProt Protein Details:

Protein type:Ribonuclease; Nucleolus; EC 3.1.13.-

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus

Molecular Function:AU-rich element binding; exoribonuclease activity; protein binding

Biological Process: defense response to virus; DNA deamination; maturation of 5.8S rRNA; positive regulation of cell growth; regulation of mRNA stability; rRNA 3'-end processing; rRNA catabolic process; rRNA processing

UniProt Code:Q9NPD3
NCBI GenInfo Identifier:14285756
NCBI Gene ID:54512
NCBI Accession:Q9NPD3.3
UniProt Related Accession:Q9NPD3
Molecular Weight:26,383 Da
NCBI Full Name:Exosome complex component RRP41
NCBI Synonym Full Names:exosome component 4
NCBI Official Symbol:EXOSC4��
NCBI Official Synonym Symbols:SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p��
NCBI Protein Information:exosome complex component RRP41
UniProt Protein Name:Exosome complex component RRP41
UniProt Synonym Protein Names:Exosome component 4; Ribosomal RNA-processing protein 41; p12A
UniProt Gene Name:EXOSC4��
UniProt Entry Name:EXOS4_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NPD3
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