Human EXOSC3 Recombinant Protein (RPPB3474)

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RPPB3474

Description

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Product Name:Human EXOSC3 Recombinant Protein
Product Code:RPPB3474
Size:5µg
Species:Human
Target:EXOSC3
Synonyms:bA3J10.7, CGI-102, hRrp-40, p10, PCH1B, RP11-3J10.8, RRP40, Rrp40p, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, EXOSC3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EXOSC3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 7.5), 0.15M NaCl, 30% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAEPASV AAESLAGSRA RAARTVLGQV VLPGEELLLP EQEDAEGPGG AVERPLSLNA RACSRVRVVC GPGLRRCGDR LLVTKCGRLR HKEPGSGSGG GVYWVDSQQK RYVPVKGDHV IGIVTAKSGD IFKVDVGGSE PASLSYLSFE GATKRNRPNV QVGDLIYGQF VVANKDMEPE MVCIDSCGRA NGMGVIGQDG LLFKVTLGLI RKLLAPDCEI IQEVGKLYPL EIVFGMNGRI WVKAKTIQQT LILANILEAC EHMTSDQRKQ IFSRLAES

Exosome Component 3 (EXOSC3) is a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity. EXOSC3 takes part in various RNA processing and degradation activities including degradation of histone mRNA. Linked pseudogenes of EXOSC3 are located on chromosome 19 and 21.

EXOSC3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275 a.a) and having a molecular mass of 32.0kDa. EXOSC3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EXOSC3: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5. Defects in EXOSC3 are the cause of pontocerebellar hypoplasia type 1B (PCH1B). A severe autosomal recessive neurologic disorder characterized by a combination of cerebellar and spinal motor neuron degeneration beginning at birth. There is diffuse muscle weakness, progressive microcephaly, global developmental delay, and brainstem involvement. Belongs to the RRP40 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.13.-; Nucleolus; RNA processing; Ribonuclease

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus

Molecular Function:exoribonuclease activity; protein binding; RNA binding

Biological Process: DNA deamination; exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); isotype switching; regulation of mRNA stability; rRNA processing

NCBI Summary:This gene encodes a non-catalytic component of the human exosome, a complex with 3'-5' exoribonuclease activity that plays a role in numerous RNA processing and degradation activities. Related pseudogenes of this gene are found on chromosome 19 and 21. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2012]
UniProt Code:Q9NQT5
NCBI GenInfo Identifier:14285758
NCBI Gene ID:51010
NCBI Accession:Q9NQT5.3
UniProt Secondary Accession:Q9NQT5,Q5QP85, Q9Y3A8, A8K0K6,
UniProt Related Accession:Q9NQT5
Molecular Weight:30kDa
NCBI Full Name:Exosome complex component RRP40
NCBI Synonym Full Names:exosome component 3
NCBI Official Symbol:EXOSC3��
NCBI Official Synonym Symbols:p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7��
NCBI Protein Information:exosome complex component RRP40
UniProt Protein Name:Exosome complex component RRP40
UniProt Synonym Protein Names:Exosome component 3; Ribosomal RNA-processing protein 40; p10
Protein Family:Exosome complex component
UniProt Gene Name:EXOSC3��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NQT5
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