Cell Death Recombinant Proteins
Human ERN1/IRE1 Recombinant Protein (RPES1048)
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- SKU:
- RPES1048
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Cell Death
Description
system_update_altDatasheet
Human ERN1/IRE1 Recombinant Protein
The encoded protein contains two functional catalytic domains; a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins; regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
| Product Name: | Human ERN1/IRE1 Recombinant Protein (RPES1048) |
| Product Code: | RPES1048 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | hIRE1p,IRE1,IRE1a,IRE1P |
| Accession: | O75460-1 |
| Sequence: | Pro 465-Leu 977 |
| Fusion tag: | N-His & GST |
| Activity: | 1.Kinase activity untested 2. The specific activity was determined to be 5 nmol/min/mg using MBP as substrate |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Purity: | > 80 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 86 kDa |
| AP Mol Mass: | 90 kDa |
| Formulation: | Supplied as sterile 20mM Tris; 500mM NaCl; 10% gly; pH 7.4 |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
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