Enzymes Recombinant Proteins

Human ErbB3 Recombinant Protein (RPPB2508)

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RPPB2508

Description

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Product Name:Human ErbB3 Recombinant Protein
Product Code:RPPB2508
Size:20µg
Species:Human
Target:ErbB3
Synonyms:Receptor tyrosine-protein kinase erbB-3, EC 2.7.10.1, c-erbB3, Tyrosine kinase-type cell surface receptor HER3, ErbB3, HER3.
Source:Escherichia Coli
Physical Appearance:A white semitransparent suspension at a concentration of 1 mg/ml.
Formulation:Each mg protein contains 1 mg aluminum hydroxide 10mM arginine, 10mM sodium chloride, 20mM sodium phosphate buffer and 5mM potassium phosphate.Dilution: It is recommended that sterile phosphate-buffered saline containing 1mg aluminum hydroxide be added to the vial to prepare a stock solution.
Stability:ErbB3 although stable at 4°C for 1 week, should be stored below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity:Measured by its ability to postpone tumor emerge time of spontaneous breast cancer in FVB/N transgenic mice and inhibit the development of tumor, effectively inhibit the growth of in situ transplanted breast cancer in FVB/N transgenic mice.

ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 can induce specific antibody production in vivo, hence to inhibit tumor cell growth. ErbB-3 can be used to treat early, medium and advanced or post-operative breast cancer with over-expression of ErbB2. According to its mechanism of action, ErbB3 is classified as a therapeutic for cancer.

Tyrosine Kinase ErbB3 Human Recombinant (HER3) produced in E.Coli is a single, non-glycosylated polypeptide consisting of several epitopes of extracellular domain of human Erb-b3, and having a total molecular mass of approximately 12.0 kDa.The ErbB3 is purified by proprietary chromatographic techniques.

UniProt Protein Function:HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Oncoprotein; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; receptor complex; lateral plasma membrane

Molecular Function:identical protein binding; protein binding; transmembrane receptor activity; protein homodimerization activity; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; protein tyrosine kinase activator activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; wound healing; heart development; cranial nerve development; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; peripheral nervous system development; negative regulation of signal transduction; regulation of cell proliferation; Schwann cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; neuron apoptosis; innate immune response; negative regulation of secretion; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2

NCBI Summary:This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:P21860
NCBI GenInfo Identifier:54792102
NCBI Gene ID:2065
NCBI Accession:NP_001005915.1
UniProt Secondary Accession:P21860,Q9BUD7, A8K6L6, B4DIK7, B4DV32, E9PDT8,
UniProt Related Accession:P21860
Molecular Weight:148,098 Da
NCBI Full Name:receptor tyrosine-protein kinase erbB-3 isoform s
NCBI Synonym Full Names:v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
NCBI Official Symbol:ERBB3��
NCBI Official Synonym Symbols:HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3��
NCBI Protein Information:receptor tyrosine-protein kinase erbB-3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3
UniProt Protein Name:Receptor tyrosine-protein kinase erbB-3
UniProt Synonym Protein Names:Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3
Protein Family:Receptor tyrosine-protein kinase
UniProt Gene Name:ERBB3��
UniProt Entry Name:ERBB3_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P21860
Research Area:
Enzymes
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