Growth Factors & Cytokines Recombinant Proteins

Human EPOR Recombinant Protein (RPPB0193)

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SKU:
RPPB0193
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P19235
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human EPOR Recombinant Protein
Product Code:RPPB0193
Size:10µg
Species:Human
Target:EPOR
Synonyms:EPO-R, EPOR, Erythropoietin Receptor.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered clear solution.
Formulation:EPOR protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by analysis by SDS-PAGE.
Amino Acid Sequence:APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGPGNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVLLDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRTRYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH
Biological Activity:Measured by its ability to inhibit EPO dependentproliferation assay using TF-1 human erythroleukemic cells. The ED50 for thiseffect less or equal to 70ng/ml.

Erythropoietin receptor, also known as EPOR arbitrates erythropoietin-induced erythroblast proliferation as well as differentiation. During EPO binding, EPOR activates Jak2 tyrosine kinase which activates various intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. Furthermore, stimulated EPOR has a function in erythroid cell survival. Mutations in EPOR may possibly produce erythroleukemia and familial erythrocytosis. In addition, dysregulation of EPOR can affect on the growth of selected tumors.

EPOR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 232 amino acids (25-250a.a.) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).EPOR is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane

Molecular Function:erythropoietin receptor activity; identical protein binding; protein binding

Disease: Erythrocytosis, Familial, 1

NCBI Summary:This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:P19235
NCBI GenInfo Identifier:119524
NCBI Gene ID:2057
NCBI Accession:P19235.1
UniProt Secondary Accession:P19235,Q15443, Q2M205, B2RCG4,
UniProt Related Accession:P19235
Molecular Weight:35,809 Da
NCBI Full Name:Erythropoietin receptor
NCBI Synonym Full Names:erythropoietin receptor
NCBI Official Symbol:EPOR��
NCBI Official Synonym Symbols:EPO-R��
NCBI Protein Information:erythropoietin receptor
UniProt Protein Name:Erythropoietin receptor
Protein Family:Erythropoietin receptor
UniProt Gene Name:EPOR��
UniProt Entry Name:EPOR_HUMAN
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