Growth Factors & Cytokines Recombinant Proteins

Human EPO a Fc Recombinant Protein (RPPB0188)

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SKU:
RPPB0188
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01588
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Human EPO a Fc Recombinant Protein
Product Code:RPPB0188
Size:10µg
Species:Human
Target:EPO a Fc
Synonyms:EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Source:Chinese Hamster Ovary Cells (CHO)
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Each mg of lyophilized powder contains 1x PBS pH-7.4.
Solubility:It is recommended to reconstitute the lyophilized Erythropoietin in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity:The ED50 as determined by the dose-dependent stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/mg.

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.

UniProt Protein Function:EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function:erythropoietin receptor binding; protein binding

Biological Process: cell proliferation; erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2

NCBI Summary:This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq, Jul 2008]
UniProt Code:P01588
NCBI GenInfo Identifier:119526
NCBI Gene ID:2056
NCBI Accession:P01588.1
UniProt Secondary Accession:P01588,Q2M2L6, Q549U2, Q9UDZ0, Q9UEZ5, Q9UHA0,
UniProt Related Accession:P01588
Molecular Weight:21kDa
NCBI Full Name:Erythropoietin
NCBI Synonym Full Names:erythropoietin
NCBI Official Symbol:EPO��
NCBI Official Synonym Symbols:EP; MVCD2��
NCBI Protein Information:erythropoietin
UniProt Protein Name:Erythropoietin
UniProt Synonym Protein Names:INN: Epoetin
Protein Family:Erythropoietin
UniProt Gene Name:EPO��
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