Cytokines Recombinant Proteins

Human Endoglin Recombinant Protein (RPPB0177)

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SKU:
RPPB0177
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17813
Research Area:
Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human Endoglin Recombinant Protein
Product Code:RPPB0177
Size:10µg
Species:Human
Target:Endoglin
Synonyms:CD105, ENG, END, ORW, HHT1, ORW1, FLJ41744, Endoglin.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Endoglin was lyophilized from a concentrated (1mg/ml) sterile solution containing 1xPBS.
Solubility:It is recommended to reconstitute the lyophilized CD-105 in sterile PBS not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Endoglin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD105 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MDRGTLPLAVALLLASCSLSPTSLAETVHCDLQPVGPERGEVTYTTSQVSKGCVAQAPNAILEVHVLFLEFPTGPSQLELTLQASKQNGTWPREVLLVLSVNSSVFLHLQALGIPLHLAYNSSLVTFQEPPGVNTTELPSFPKTQILEWAAERGPITSAAELNDPQSILLRLGQAQGSLSFCMLEASQDMGRTLEWRPRTPALVRGCHLEGVAGHKEAHILRVLPGHSAGPRTVTVKVELSCAPGDLDAVLILQGPPYVSWLIDANHNMQIWTTGEYSFKIFPEKNIRGFKLPDTPQGLLGEARMLNASIVASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRKKVHCLNMDSLSFQLGLYLSPHFLQASNTIEPGQQSFVQVRVSPSVSEFLLQLDSCHLDLGPEGGTVELIQGRAAKGNCVSLLSPSPEGDPRFSFLLHFYTVPIPKTGTLSCTVALRPKTGS
Biological Activity:Measured by its ability to bind with rhTGF-beta RII/Fc in a functional ELISA. Optimal dilutions should be determined by each laboratory for each application.

Endoglin is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex.The protein consists of a homodimer of 180 kDA with disulfide links. It has been found on endothelial cells, activated macrophages, fibroblasts, and smooth muscle cells. Endoglin has been found to be part of the TGF-beta1 receptor complex. It thus may be involved in the binding of TGF-beta1, TGF-beta3, activin-A, BMP-2, and BMP-7. Beside TGF-beta signaling endoglin may have other functions. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development . Experimental mice without the endoglin gene die due to cardiovascular abnormalities.

CD105 Human Recombinant extracellular domain produced in baculovirus is a homodimeric, glycosylated, Polypeptide containing 586 amino acids and having a molecular mass of 61 kDa but as a result of glycosylation, migrates at 90 kDa under reducing conditions in SDS-PAGE.The CD105 is fused to a C-terminal His-tag (6xHis) and purified by proprietary chromatographic techniques.

UniProt Protein Function:ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; focal adhesion; nucleoplasm; receptor complex

Molecular Function:activin binding; galactose binding; glycosaminoglycan binding; protein binding; punt binding; transforming growth factor beta binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: artery morphogenesis; BMP signaling pathway; central nervous system vasculogenesis; heart looping; negative regulation of cell migration; negative regulation of protein amino acid autophosphorylation; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of protein amino acid phosphorylation; regulation of transcription, DNA-dependent; regulation of transforming growth factor beta receptor signaling pathway; smooth muscle development; vasculogenesis; venous blood vessel morphogenesis

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber

NCBI Summary:This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
UniProt Code:P17813
NCBI GenInfo Identifier:3041681
NCBI Gene ID:2022
NCBI Accession:P17813.2
UniProt Secondary Accession:P17813,Q14248, Q14926, Q5T9C0,
UniProt Related Accession:P17813
Molecular Weight:67,542 Da
NCBI Full Name:Endoglin
NCBI Synonym Full Names:endoglin
NCBI Official Symbol:ENG��
NCBI Official Synonym Symbols:END; HHT1; ORW1��
NCBI Protein Information:endoglin
UniProt Protein Name:Endoglin
UniProt Synonym Protein Names:CD_antigen: CD105
Protein Family:Endoglin
UniProt Gene Name:ENG��
UniProt Entry Name:EGLN_HUMAN
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system_update_altDatasheetHuman Endoglin/CD105 Recombinant Protein Endoglin is a single-pass type I membrane protein which restricted to endothelial cells in all tissues except bone marrow. Endog