Human EIF5A Recombinant Protein (RPPB3437)
- SKU:
- RPPB3437
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63241
Description
| Product Name: | Human EIF5A Recombinant Protein |
| Product Code: | RPPB3437 |
| Size: | 20µg |
| Species: | Human |
| Target: | EIF5A |
| Synonyms: | EIF-5A, EIF5A1, eIF5AI, MGC99547, MGC104255, EIF5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-4D, Rev-binding factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The EIF5A protein solution (1mg/ml) contains 50mM Tris-HCl, pH-7.5 and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK |
EIF5A is the single protein identified to contain remarkable amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. EIF5A takes part in the first step of peptide bond formation in translation, nevertheless further experiments implicates it as a universally conserved translation elongation factor. Modulation of EIF5A is connected to proliferation and cancer. Expression of EIF-5A is upregulated in the PBMCs of HIV-1 patients. EIF5A coordinates significant cellular processes like cell viability and senescence during its effects on the stability of certain mRNAs. Heat stress-induced loss of EIF-5A in a human pancreatic cancer cell line. EIF5A stability takes part in determining the fate of the particular cell type after severe heat stress.
EIF5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids and having a molecular mass of 16.8 kDa.EIF5A is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 17p13-p12 Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; cytosol; nucleus; annulate lamellae Molecular Function:protein binding; RNA binding; ribosome binding; protein N-terminus binding; translation elongation factor activity; U6 snRNA binding Biological Process: mRNA export from nucleus; translational frameshifting; cellular protein metabolic process; apoptosis; positive regulation of cell proliferation; nucleocytoplasmic transport; positive regulation of translational elongation; hypusine biosynthetic process from peptidyl-lysine; protein export from nucleus; post-translational protein modification; positive regulation of translational termination |
| UniProt Code: | P63241 |
| NCBI GenInfo Identifier: | 54037409 |
| NCBI Gene ID: | 1984 |
| NCBI Accession: | P63241.2 |
| UniProt Related Accession: | P63241 |
| Molecular Weight: | ~ 18kDa |
| NCBI Full Name: | Eukaryotic translation initiation factor 5A-1 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 5A |
| NCBI Official Symbol: | EIF5A�� |
| NCBI Official Synonym Symbols: | EIF-5A; EIF5A1; eIF-4D; eIF5AI�� |
| NCBI Protein Information: | eukaryotic translation initiation factor 5A-1 |
| UniProt Protein Name: | Eukaryotic translation initiation factor 5A-1 |
| UniProt Synonym Protein Names: | Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D |
| Protein Family: | Translation initiation factor |
| UniProt Gene Name: | EIF5A�� |
| UniProt Entry Name: | IF5A1_HUMAN |
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