Human EIF4H Recombinant Protein (RPPB3436)

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RPPB3436

Description

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Product Name:Human EIF4H Recombinant Protein
Product Code:RPPB3436
Size:20µg
Species:Human
Target:EIF4H
Synonyms:Eukaryotic translation initiation factor 4H, eIF-4H, Williams-Beuren syndrome chromosomal region 1 protein, EIF4H, KIAA0038, WBSCR1, WSCR1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EIF4H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE

Eukaryotic translation initiation factor 4H (EIF4H) is a 248 amino acid protein which localizes to the perinuclear region of the cytoplasm and is expressed as 2 isoforms, termed short and long. EIF4H functions to stimulate the initiation of protein synthesis at the level of mRNA employment. EIF4H stimulates the RNA-dependent ATP hydrolysis catalyzed by EIF4A and EIF4B. EIF4H gene defects linked to Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities and caused by the deletion of contiguous genes at 7q11.23.

EIF4H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 272 amino acids (1-248 a.a) and having a molecular mass of 29.9kDa.EIF4H is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
UniProt Protein Details:

Protein type:Translation initiation; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; membrane; polysome

Molecular Function:helicase activity; protein binding; ribosomal small subunit binding; RNA binding; RNA strand annealing activity; RNA strand-exchange activity; translation factor activity, nucleic acid binding

Biological Process: formation of translation preinitiation complex; regulation of translational initiation; translational initiation

Disease: Williams-beuren Syndrome

NCBI Summary:This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:Q15056
NCBI GenInfo Identifier:18276665
NCBI Gene ID:7458
NCBI Accession:Q15056.5
UniProt Secondary Accession:Q15056,A8K3R1, D3DXF6, D3DXF8,
UniProt Related Accession:Q15056
Molecular Weight:25,200 Da
NCBI Full Name:Eukaryotic translation initiation factor 4H
NCBI Synonym Full Names:eukaryotic translation initiation factor 4H
NCBI Official Symbol:EIF4H��
NCBI Official Synonym Symbols:WSCR1; WBSCR1; eIF-4H��
NCBI Protein Information:eukaryotic translation initiation factor 4H
UniProt Protein Name:Eukaryotic translation initiation factor 4H
UniProt Synonym Protein Names:Williams-Beuren syndrome chromosomal region 1 protein
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF4H��
UniProt Entry Name:IF4H_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15056
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