Human EIF3K Recombinant Protein (RPPB3429)

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SKU:
RPPB3429
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UBQ5

Description

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Product Name:Human EIF3K Recombinant Protein
Product Code:RPPB3429
Size:10µg
Species:Human
Target:EIF3K
Synonyms:Eukaryotic Translation Initiation Factor 3 Subunit K, PLAC-24, eIF3k, eIF-3 p25, eIF-3 p28, EIF3S12, HSPC029, M9, PRO1474, PTD001, Muscle-specific gene M9 protein, ARG134, MSTP001, muscle specific, Eukaryotic Translation Initiation Factor 3 subunit 12.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The EIF3K protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY QHIDRWLLAE MLGDLSDSQL KVWMSKYGWS ADESGQIFIC SQEESIKPKN IVEKIDFDSV SSIMASSQ

EIF3K is a member of the eIF3 subunit K family. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 40S ribosomal subunit. EIF3K is conserved among high eukaryotes, including mammals, insects, and plants, and it is universally expressed in human tissues. EIF3K is distributed both in nucleus and cytoplasm and colocalized with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4).

EIF3K produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-218a.a.) and having a molecular mass of 27.2 kDa. EIF3K is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:EIF3K: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Belongs to the eIF-3 subunit K family.
UniProt Protein Details:

Protein type:Translation initiation; Translation

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane

Molecular Function:protein binding; translation initiation factor activity

Biological Process: translational initiation

NCBI Summary:The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9UBQ5
NCBI GenInfo Identifier:23396628
NCBI Gene ID:27335
NCBI Accession:Q9UBQ5.1
UniProt Secondary Accession:Q9UBQ5,Q96IQ0, Q9Y6D1, A8K0I9, B7ZAM9,
UniProt Related Accession:Q9UBQ5
Molecular Weight:24,484 Da
NCBI Full Name:Eukaryotic translation initiation factor 3 subunit K
NCBI Synonym Full Names:eukaryotic translation initiation factor 3 subunit K
NCBI Official Symbol:EIF3K��
NCBI Official Synonym Symbols:M9; ARG134; PLAC24; PTD001; EIF3S12; HSPC029; MSTP001; PLAC-24; PRO1474; EIF3-p28��
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit K
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit K
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 3 subunit 12
UniProt Gene Name:EIF3K��
UniProt Entry Name:EIF3K_HUMAN
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