Human EIF3F Recombinant Protein (RPPB3425)

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SKU:
RPPB3425
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00303

Description

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Product Name:Human EIF3F Recombinant Protein
Product Code:RPPB3425
Size:20µg
Species:Human
Target:EIF3F
Synonyms:Eukaryotic Translation Initiation Factor 3, Subunit F, EIF3S5 , Eukaryotic Translation Initiation Factor 3, Subunit 5 (Epsilon, 47kD), EC 3.4.19.12, Deubiquitinating Enzyme EIF3f, eIF3f, eIF-3-epsilon, EIF3 P47, Eukaryotic Translation Initiation Factor 3 Subunit 5, eIF3-p47, Eukaryotic Translation Initiation Factor 3, Subunit 5 Epsilon, 47kDa.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:EIF3F protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, and 0.4M urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL

Eukaryotic Translation Initiation Factor 3F, alsoknown as EIF3F belongs to the eIF-3 subunit F family. EIF3F is a component ofthe eukaryotic translation initiation factor 3 (eIF-3) complex, which is essential for several steps in the initiation of proteinsynthesis. The eIF-3 complex associates with the 40S ribosome andfacilitates the recruitment of eIF-1, eIF-1A, eIF 2: GTP: methionyl-tRNAi andeIF-5 to form the 43S preinitiation complex (43S PIC). Inaddition, the eIF-3 complex stimulates mRNA recruitment to the 43S PIC andscanning of the mRNA for AUG recognition. TheeIF-3 complex is also necessary for disassembly and recycling of posttermination ribosomal complexes and subsequently preventspremature union of the 40S and 60S ribosomal subunits prior to initiation. Among the diseases associated with EIF3F are intrahepaticcholangiocarcinoma, and cholangiocarcinoma.

EIF3F Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-357 a.a) and having a molecular mass of 40kDa. EIF3F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
UniProt Code:O00303
NCBI GenInfo Identifier:4503519
NCBI Gene ID:8665
NCBI Accession:NP_003745.1
UniProt Secondary Accession:O00303,Q6IB45, Q8N978, A8K0S2,
UniProt Related Accession:O00303
Molecular Weight:38 KD
NCBI Full Name:eukaryotic translation initiation factor 3 subunit F
NCBI Synonym Full Names:eukaryotic translation initiation factor 3 subunit F
NCBI Official Symbol:EIF3F��
NCBI Official Synonym Symbols:EIF3S5; eIF3-p47��
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit F
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit F
UniProt Synonym Protein Names:Deubiquitinating enzyme eIF3f (EC:3.4.19.12); Eukaryotic translation initiation factor 3 subunit 5UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF-3-epsilonUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005; eIF3 p47UniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03005
UniProt Gene Name:EIF3F��
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