Growth Factors & Cytokines Recombinant Proteins

Human EGF Recombinant Protein (RPPB0163)

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SKU:
RPPB0163
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01133
Research Area:
Growth Factors & Cytokines

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human EGF Recombinant Protein
Product Code:RPPB0163
Size:0.5mg
Species:Human
Target:EGF
Synonyms:Urogastrone, URG, EGF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.
Solubility:It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Amino Acid Sequence:NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Biological Activity:The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6.2kDa. The EGF is purified by proprietary chromatographic techniques.

NCBI Summary:This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
UniProt Code:P01133
NCBI GenInfo Identifier:251757262
NCBI Gene ID:1950
NCBI Accession:P01133.2
UniProt Related Accession:P01133
Molecular Weight:6.2
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:EGF��
NCBI Official Synonym Symbols:URG; HOMG4��
NCBI Protein Information:pro-epidermal growth factor
UniProt Protein Name:Pro-epidermal growth factor
UniProt Synonym Protein Names:Urogastrone
Protein Family:Epidermal growth factor
UniProt Gene Name:EGF��
UniProt Entry Name:EGF_HUMAN
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