Human ECSIT Recombinant Protein (RPPB3387)

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SKU:
RPPB3387
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BQ95

Description

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Product Name:Human ECSIT Recombinant Protein
Product Code:RPPB3387
Size:10µg
Species:Human
Target:ECSIT
Synonyms:ECSIT Homolog (Drosophila), Evolutionarily Conserved Signaling Intermediate In Toll Pathway Mitochondrial, Likely Ortholog Of Mouse Signaling Intermediate In Toll Pathway Evolutionarily Conserved, Protein SITPEC.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ECSIT solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS

ECSIT homolog (ECSIT) is a ubiquitously expressed protein which has a vital role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. ECSIT promotes proteolytic activation of MAP3K1. ECSIT is also involved in the BMP signaling pathway. ECSIT is essential for normal embryonic development. ECSIT was originally classified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. ECSIT gene knockdown results in gravely impaired complex I assembly and disrupted mitochondrial function.

ECSIT Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (19-217) and having a molecular mass of 24.6 kDa.ECSIT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ECSIT: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Belongs to the ECSIT family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleoplasm; nucleus

Molecular Function:oxidoreductase activity, acting on NADH or NADPH; protein binding

Biological Process: mitochondrial respiratory chain complex I assembly; regulation of oxidoreductase activity

UniProt Code:Q9BQ95
NCBI GenInfo Identifier:74752234
NCBI Gene ID:51295
NCBI Accession:Q9BQ95.1
UniProt Secondary Accession:Q9BQ95,Q96HQ7, Q9NYI1, E9PAN9, K7EMM0,
UniProt Related Accession:Q9BQ95
Molecular Weight:33,938 Da
NCBI Full Name:Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
NCBI Synonym Full Names:ECSIT signalling integrator
NCBI Official Symbol:ECSIT��
NCBI Official Synonym Symbols:SITPEC��
NCBI Protein Information:evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
UniProt Protein Name:Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
UniProt Synonym Protein Names:Protein SITPEC
Protein Family:Evolutionarily conserved signaling intermediate
UniProt Gene Name:ECSIT��
UniProt Entry Name:ECSIT_HUMAN
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