Enzymes Recombinant Proteins

Human ECHS1 Recombinant Protein (RPPB1607)

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SKU:
RPPB1607
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P30084
Research Area:
Enzymes

Description

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Product Name:Human ECHS1 Recombinant Protein
Product Code:RPPB1607
Size:10µg
Species:Human
Target:ECHS1
Synonyms:Enoyl-CoA Hydratase, Short Chain1, Enoyl Coenzyme A Hydratase, Short Chain, 1, Mitochondrial, Enoyl-CoA Hydratase, Short Chain, 1, Mitochondrial, Short Chain Enoyl-CoA Hydratase, EC 4.2.1.17, SCEH, Enoyl-CoA Hydratase, Mitochondrial, Short-Chain Enoyl-CoA Hydratase, Enoyl-CoA Hydratase 1, EC 4.2.1, ECHS1D, ECHS1�.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:ECHS1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% Glycerol and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
Biological Activity:Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25�C.

ECHS1 is part of the hydratase/isomerase superfamily. ECHS1 is localized in the mitochondrial matrix and Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that takes part in the second phase of the mitochondrial fatty acid Beta-oxidation pathway.

ECHS1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (28-290 a.a) and having a molecular mass of 30.6kDa. ECHS1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
NCBI Summary:The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]
UniProt Code:P30084
NCBI GenInfo Identifier:62906863
NCBI Gene ID:1892
NCBI Accession:P30084.4
UniProt Secondary Accession:P30084,O00739, Q5VWY1, Q96H54,
UniProt Related Accession:P30084
Molecular Weight:31,387 Da
NCBI Full Name:Enoyl-CoA hydratase, mitochondrial
NCBI Synonym Full Names:enoyl-CoA hydratase, short chain 1
NCBI Official Symbol:ECHS1��
NCBI Official Synonym Symbols:SCEH; ECHS1D��
NCBI Protein Information:enoyl-CoA hydratase, mitochondrial
UniProt Protein Name:Enoyl-CoA hydratase, mitochondrial
UniProt Synonym Protein Names:Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH
Protein Family:Enoyl-CoA hydratase
UniProt Gene Name:ECHS1��
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