Description
| Product Name: | Human DUSP3 Recombinant Protein |
| Product Code: | RPPB1601 |
| Size: | 20µg |
| Species: | Human |
| Target: | DUSP3 |
| Synonyms: | Dual specificity phosphatase 3, VHR, Vaccinia virus phosphatase VH1-related, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, serine/threonine specific protein phosphatase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DUSP3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP |
| Biological Activity: | Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. |
DUSP3 belongs to the dual specificity protein phosphatase subfamily which inactivates their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSP3 negatively regulate members of the mitogen-activated protein kinase superfamily that are related to cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues and displays activity both for tyrosine-protein phosphate and serine-protein phosphate, but exhibits a strong preference toward phosphotyrosines, specifically dephosphorylates and inactivates ERK1 and ERK2.
DUSP3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 205 amino acids (1-185) and having a molecular mass of 22.6 kDa.DUSP3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway. |
| UniProt Protein Details: | Protein type:EC 3.1.3.48; EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity Chromosomal Location of Human Ortholog: 17q21 Cellular Component: nucleoplasm; cytoplasm; immunological synapse; nucleus; cytosol Molecular Function:MAP kinase phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity Biological Process: negative regulation of JNK cascade; nerve growth factor receptor signaling pathway; in utero embryonic development; negative regulation of MAPKKK cascade; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; negative regulation of T cell receptor signaling pathway; toll-like receptor 3 signaling pathway; positive regulation of mitotic cell cycle; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; negative regulation of T cell activation; inactivation of MAPK activity |
| NCBI Summary: | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P51452 |
| NCBI GenInfo Identifier: | 1718191 |
| NCBI Gene ID: | 1845 |
| NCBI Accession: | P51452.1 |
| UniProt Secondary Accession: | P51452,Q5U0J1, Q8IYJ9, D3DX45, |
| UniProt Related Accession: | P51452 |
| Molecular Weight: | 16,163 Da |
| NCBI Full Name: | Dual specificity protein phosphatase 3 |
| NCBI Synonym Full Names: | dual specificity phosphatase 3 |
| NCBI Official Symbol: | DUSP3�� |
| NCBI Official Synonym Symbols: | VHR�� |
| NCBI Protein Information: | dual specificity protein phosphatase 3; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase |
| UniProt Protein Name: | Dual specificity protein phosphatase 3 |
| UniProt Synonym Protein Names: | Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR |
| Protein Family: | Dual specificity protein phosphatase |
| UniProt Gene Name: | DUSP3�� |
| UniProt Entry Name: | DUS3_HUMAN |
