Human DR1 Recombinant Protein (RPPB3375)
- SKU:
- RPPB3375
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q01658
Description
| Product Name: | Human DR1 Recombinant Protein |
| Product Code: | RPPB3375 |
| Size: | 25µg |
| Species: | Human |
| Target: | DR1 |
| Synonyms: | NC2, NC2-BETA, Negative cofactor 2-beta. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | DR1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.1mM PMSF, 0.1M NaCl & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA NQAGSSQDE EDDDDI |
DR1 is a phosphoprotein that inhibits both basal and activated levels of transcription. DR1 is phosphorylated in vivo which affects its interaction with TBP. DR1 has a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 alters the physiological balance of transcriptional output in favor of polymerase I.
DR1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-176 a.a.) and having a molecular mass of 21.6 kDa. The DR1 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DR1: The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Heterodimer with DRAP1. DR1 exists in solution as a homotetramer that dissociates during interaction with TBP and then, after complexing with TBP, reassociates at a slow rate, to reconstitute the tetramer. Interacts with NFIL3. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Belongs to the NC2 beta/DR1 family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1p22.1 Cellular Component: nucleus Molecular Function:protein binding; TATA-binding protein binding; transcription corepressor activity; transcription factor binding Biological Process: negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q01658 |
| NCBI GenInfo Identifier: | 401162 |
| NCBI Gene ID: | 1810 |
| NCBI Accession: | Q01658.1 |
| UniProt Related Accession: | Q01658 |
| Molecular Weight: | 19,444 Da |
| NCBI Full Name: | Protein Dr1 |
| NCBI Synonym Full Names: | down-regulator of transcription 1 |
| NCBI Official Symbol: | DR1�� |
| NCBI Official Synonym Symbols: | NC2; NC2B; NC2-BETA�� |
| NCBI Protein Information: | protein Dr1 |
| UniProt Protein Name: | Protein Dr1 |
| UniProt Synonym Protein Names: | Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein |
| Protein Family: | Dr1-associated corepressor |
| UniProt Gene Name: | DR1�� |
| UniProt Entry Name: | NC2B_HUMAN |
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