Human DNAJC15 Recombinant Protein (RPPB5206)

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SKU:
RPPB5206
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y5T4

Description

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Product Name:Human DNAJC15 Recombinant Protein
Product Code:RPPB5206
Size:10µg
Species:Human
Target:DNAJC15
Synonyms:DnaJ homolog subfamily C member 15, Cell growth-inhibiting gene 22 protein, Methylation-controlled J protein, MCJ, DNAJC15, DNAJD1, GIG22, HSD18.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DNAJC15 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSFRIWKPL EQVITETAKK ISTPSFSSYY KGGFEQKMSR REAGLILGVS PSAGKAKIRT AHRRVMILNH PDKGGSPYVA AKINEAKDLL ETTTKH

DNAJC15 which is expressed ubiquitously and located on the membrane contains 1 J domain. In many advanced cases of ovarian adenocarcinoma DNAJC15 is absent or down-regulated, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin. DNAJC15is a crucial component of the TIM23 translocase complex and stimulates the ATPase activity of HSPA9.

DNAJC15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 116 amino acids (58-150 a.a.) and having a molecular mass of 12.8 kDa.DNAJC15 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DNAJC15: Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP. Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9. Interacts with the TIM23 complex. Directly interacts with PAM16/MAGMAS; this interaction counteracts DNAJC15-dependent stimulation of HSPA9 ATPase activity. Associates with complex I of the mitochondrial electron transfer chain; this interaction may interfere with the formation of supercomplexes that facilitate the transfer of electrons between complexes
UniProt Protein Details:

Protein type:Chaperone; Membrane protein, integral; Cell adhesion; Mitochondrial

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: protein transport; regulation of lipid metabolic process; cellular response to starvation; negative regulation of protein complex assembly

UniProt Code:Q9Y5T4
NCBI GenInfo Identifier:110808202
NCBI Gene ID:29103
NCBI Accession:Q9Y5T4.2
UniProt Secondary Accession:Q9Y5T4,Q5T219, Q6X963, B2R4L0,
UniProt Related Accession:Q9Y5T4
Molecular Weight:16,383 Da
NCBI Full Name:DnaJ homolog subfamily C member 15
NCBI Synonym Full Names:DnaJ (Hsp40) homolog, subfamily C, member 15
NCBI Official Symbol:DNAJC15��
NCBI Official Synonym Symbols:MCJ; HSD18; DNAJD1��
NCBI Protein Information:dnaJ homolog subfamily C member 15; DNAJ domain-containing; DnaJ (Hsp40) homolog, subfamily D, member 1; cell growth-inhibiting gene 22 protein; methylation-controlled J protein
UniProt Protein Name:DnaJ homolog subfamily C member 15
UniProt Synonym Protein Names:Cell growth-inhibiting gene 22 protein; Methylation-controlled J protein; MCJ
Protein Family:DnaJ homolog subfamily
UniProt Gene Name:DNAJC15��
UniProt Entry Name:DJC15_HUMAN
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