Enzymes Recombinant Proteins
Human DHODH Recombinant Protein (RPPB1572)
- SKU:
- RPPB1572
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q02127
- Research Area:
- Enzymes
Description
| Product Name: | Human DHODH Recombinant Protein |
| Product Code: | RPPB1572 |
| Size: | 5µg |
| Species: | Human |
| Target: | DHODH |
| Synonyms: | Dihydroorotate dehydrogenase (quinone), Dihydroorotate oxidase, human complement of yeast URA1, DHOdehase, POADS, EC 1.3.5.2, EC 1.3.3.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The DHODH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR |
Dihydroorotate dehydrogenase quinone (DHODH) is a member of the dihydroorotate dehydrogenase family. DHODH is a mitochondrial protein found on the outer surface of the inner mitochondrial membrane. DHODH catalyzes the 4th enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate (with quinone as electron acceptor), in de novo pyrimidine biosynthesis. DHODH gene defects cause the postaxial acrofacial dysostosis (POADS), also known as Miller syndrome.
DHODH Human Recombinant produced in E. coli is a single polypeptide chain containing 390 amino acids (31-395) and having a molecular mass of 42.3 kDa.DHODH is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DHODH: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS); also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases. Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 1.3.5.2; Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 16q22 Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleoplasm Molecular Function:dihydroorotate dehydrogenase activity Biological Process: 'de novo' pyrimidine base biosynthetic process; pyrimidine nucleoside biosynthetic process Disease: Postaxial Acrofacial Dysostosis |
| NCBI Summary: | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02127 |
| NCBI GenInfo Identifier: | 56405372 |
| NCBI Gene ID: | 1723 |
| NCBI Accession: | Q02127.3 |
| UniProt Secondary Accession: | Q02127,Q6P176, A8K8C8, |
| UniProt Related Accession: | Q02127 |
| Molecular Weight: | 42,867 Da |
| NCBI Full Name: | Dihydroorotate dehydrogenase (quinone), mitochondrial |
| NCBI Synonym Full Names: | dihydroorotate dehydrogenase (quinone) |
| NCBI Official Symbol: | DHODH�� |
| NCBI Official Synonym Symbols: | URA1; POADS; DHOdehase�� |
| NCBI Protein Information: | dihydroorotate dehydrogenase (quinone), mitochondrial |
| UniProt Protein Name: | Dihydroorotate dehydrogenase (quinone), mitochondrial |
| UniProt Synonym Protein Names: | Dihydroorotate oxidase |
| UniProt Gene Name: | DHODH�� |
| UniProt Entry Name: | PYRD_HUMAN |
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