Enzymes Recombinant Proteins

Human DHFR Recombinant Protein (RPPB5810)

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SKU:
RPPB5810
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00374
Research Area:
Enzymes

Description

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Product Name:Human DHFR Recombinant Protein
Product Code:RPPB5810
Size:50µg
Species:Human
Target:DHFR
Synonyms:Dihydrofolate reductase, DHFR, DHFRP1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DHFR solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 2mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
Biological Activity:Specific activity is >2000 pmol/min/ug �is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Dihydrofolate reductase (DHFR) is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, with NADPH as electron donor that can be converted to the kinds of tetrahydrofolate cofactors applied in 1-carbon transfer chemistry. DHFR converts dihydrofolate into tetrahydrofolate, which is a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and specific amino acids. Even though the functional DHFR gene is mapped to chromosome 5, numerous intronless processed pseudogenes or dihydrofolate reductase-like genes are identified on separate chromosomes. DHFR deficiency is associated with megaloblastic anemia.DHFR knockdown plays a role in the anticancer activity of 2-hydroxyoleic acid.DHFR gene insertion/deletion polymorphism is linked to variation in serum and red blood cell folate concentrations in women.

DHFR Human Recombinant fused with a 20 amino acids His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.6kDa.The DHFR is purified by proprietary chromatographic techniques.

UniProt Protein Function:DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.3; Cofactor and Vitamin Metabolism - folate biosynthesis

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytosol

Molecular Function:mRNA binding; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; vitamin metabolic process; one-carbon compound metabolic process; nitric oxide metabolic process; G1/S-specific transcription in mitotic cell cycle; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; response to methotrexate; regulation of nitric-oxide synthase activity; folic acid metabolic process; mitotic cell cycle; glycine biosynthetic process; water-soluble vitamin metabolic process; G1/S transition of mitotic cell cycle

Disease: Megaloblastic Anemia Due To Dihydrofolate Reductase Deficiency

UniProt Code:P00374
NCBI GenInfo Identifier:118992
NCBI Gene ID:
NCBI Accession:P00374.2
Molecular Weight:
NCBI Full Name:Dihydrofolate reductase
UniProt Protein Name:Dihydrofolate reductase
Protein Family:Viral dihydrofolate reductase
UniProt Gene Name:DHFR��
UniProt Entry Name:DYR_HUMAN
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