Human DDIT4 Recombinant Protein (RPPB3344)

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SKU:
RPPB3344
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NX09

Description

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Product Name:Human DDIT4 Recombinant Protein
Product Code:RPPB3344
Size:10µg
Species:Human
Target:DDIT4
Synonyms:DNA-damage-inducible transcript 4, RTP801, REDD-1, Dig2, FLJ20500, Protein regulated in development and DNA damage response 1, HIF-1 responsive protein RTP801, RP11-442H21.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The DDIT4 protein solution (0.25mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC

DDIT4 has a role in the regulation of reactive oxygen species. DDIT4 is upregulated at the transcriptional level as a reaction to stress due to DNA damage and glucocorticoid treatment. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase frequently referred to as mTOR. DDIT4 is vital in the coupling of extra- and intracellular cues to mTOR regulation.

DDIT4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232.a.a) and having a molecular mass of 27.5kDa. DDIT4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytoplasm; cytosol; intracellular

Biological Process: brain development; cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of peptidyl-serine phosphorylation; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; neuron differentiation; neuron migration; response to hypoxia

UniProt Code:Q9NX09
NCBI GenInfo Identifier:74753036
NCBI Gene ID:54541
NCBI Accession:Q9NX09.1
UniProt Secondary Accession:Q9NX09,Q9H0S3,
UniProt Related Accession:Q9NX09
Molecular Weight:25,371 Da
NCBI Full Name:DNA damage-inducible transcript 4 protein
NCBI Synonym Full Names:DNA damage inducible transcript 4
NCBI Official Symbol:DDIT4��
NCBI Official Synonym Symbols:Dig2; REDD1; REDD-1��
NCBI Protein Information:DNA damage-inducible transcript 4 protein
UniProt Protein Name:DNA damage-inducible transcript 4 protein
UniProt Synonym Protein Names:HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1
Protein Family:DNA damage-inducible transcript 4 protein
UniProt Gene Name:DDIT4��
UniProt Entry Name:DDIT4_HUMAN
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