Human DCTN2 (1-406) Recombinant Protein (RPPB3338)

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SKU:
RPPB3338
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q13561

Description

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Product Name:Human DCTN2 (1-406) Recombinant Protein
Product Code:RPPB3338
Size:20µg
Species:Human
Target:DCTN2 (1-406)
Synonyms:DCTN2, Dynactin 2 (P50), DCTN50, Dynactin Complex 50 KDa Subunit, 50 KDa Dynein-Associated Polypeptide, P50 Dynamitin, 50 KD Dynein-Associated Polypeptide, DYNAMITIN, HEL-S-77, RBP50, Dynactin Complex 50 KD Subunit, Dynactin Subunit 2, Epididymis Secretory Protein Li 77, DCTN-50.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:DCTN2 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0), 20% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAFA QELEELTSTS VEHIIVNPNA AYDKFKDKRV GTKGLDFSDR IGKTKRTGYE SGEYEMLGEG LGVKETPQQK YQRLLHEVQE LTTEVEKIKT TVKESATEEK LTPVLLAKQL AALKQQLVAS HLEKLLGPDA AINLTDPDGA LAKRLLLQLE ATKNSKGGSG GKTTGTPPDS SLVTYELHSR PEQDKFSQAA KVAELEKRLT ELETAVRCDQ DAQNPLSAGL QGACLMETVE LLQAKVSALD LAVLDQVEAR LQSVLGKVNE IAKHKASVED ADTQSKVHQL YETIQRWSPI ASTLPELVQR LVTIKQLHEQ AMQFGQLLTH LDTTQQMIAN SLKDNTTLLT QVQTTMRENL ATVEGNFASI DERMKKLGK

DCTN2 is a 50kDa subunit of dynactin, which is a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a various cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. The DCTN2 subunit is present in 4-5 copies per dynactin molecule. DCTN2 is comprised of 3 short alpha-helical coiled-coil domains which mediate association with self or other dynactin subunits. DCTN2 interacts directly with the largest subunit (p150) of dynactin and is able to affix p150 in place. DCTN2 modulates cytoplasmic dynein binding to an organelle, and plays a part in prometaphase chromosome alignment and spindle organization during mitosis. DCTN2 is involved in anchoring microtubules to centrosomes. DCTN2 has a role in synapse formation during brain development.

Dynactin 2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 429 amino acids (1-406 a.a) and having a molecular mass of 47.2kDa.DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:dynactin 2: Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development. Belongs to the dynactin subunit 2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cell cycle regulation; Motor; Microtubule-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: kinetochore; dynein complex; centrosome; microtubule; dynactin complex; growth cone; membrane; cytoplasm; cytosol; vesicle

Molecular Function:protein binding; spectrin binding; motor activity

Biological Process: cell proliferation; mitosis; mitotic spindle organization and biogenesis; metabolic process; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; mitotic cell cycle; G2/M transition of mitotic cell cycle; melanosome transport

NCBI Summary:This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q13561
NCBI GenInfo Identifier:22096346
NCBI Gene ID:10540
NCBI Accession:Q13561.4
UniProt Secondary Accession:Q13561,Q86YN2, Q9BW17, B2RBK5,
UniProt Related Accession:Q13561
Molecular Weight:401
NCBI Full Name:Dynactin subunit 2
NCBI Synonym Full Names:dynactin 2 (p50)
NCBI Official Symbol:DCTN2��
NCBI Official Synonym Symbols:RBP50; DCTN50; HEL-S-77; DYNAMITIN��
NCBI Protein Information:dynactin subunit 2; p50 dynamitin; dynactin complex 50 kD subunit; dynactin complex 50 kDa subunit; epididymis secretory protein Li 77; 50 kD dynein-associated polypeptide; 50 kDa dynein-associated polypeptide
UniProt Protein Name:Dynactin subunit 2
UniProt Synonym Protein Names:50 kDa dynein-associated polypeptide; Dynactin complex 50 kDa subunit; DCTN-50; p50 dynamitin
Protein Family:Dynactin
UniProt Gene Name:DCTN2��
UniProt Entry Name:DCTN2_HUMAN
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