Enzymes Recombinant Proteins

Human DCTD Recombinant Protein (RPPB1558)

(No reviews yet) Write a Review
SKU:
RPPB1558

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human DCTD Recombinant Protein
Product Code:RPPB1558
Size:25µg
Species:Human
Target:DCTD
Synonyms:Deoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ

DCTD is an allosteric enzyme that exists as a homohexamer and is part of the cytidine and deoxycytidylate deaminase protein family. DTCD uses zinc as a cofactor to catalyze the deamination of dCMP to dUMP, thus making the nucleotide substrate (dUMP) that is used by thymidylate synthase.

DCTD Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:DCTD: catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.
UniProt Protein Details:

Protein type:Nucleotide Metabolism - pyrimidine; EC 3.5.4.12; Hydrolase

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cytosol

Molecular Function:zinc ion binding; dCMP deaminase activity

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleotide biosynthetic process; pyrimidine nucleotide metabolic process; pyrimidine nucleoside biosynthetic process

NCBI Summary:The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P32321
NCBI GenInfo Identifier:23503055
NCBI Gene ID:1635
NCBI Accession:P32321.2
UniProt Secondary Accession:P32321,Q5M7Z8, Q9BVD8, B2R836, D3DP49, D3DP50,
UniProt Related Accession:P32321
Molecular Weight:178
NCBI Full Name:Deoxycytidylate deaminase
NCBI Synonym Full Names:dCMP deaminase
NCBI Official Symbol:DCTD��
NCBI Protein Information:deoxycytidylate deaminase
UniProt Protein Name:Deoxycytidylate deaminase
UniProt Synonym Protein Names:dCMP deaminase
Protein Family:Deoxycytidylate deaminase
UniProt Gene Name:DCTD��
UniProt Entry Name:DCTD_HUMAN
View AllClose

Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P32321
Research Area:
Enzymes
View AllClose

Related Products