ACE (Angiotensin I Converting Enzyme) inhibitors are a type of medication used to treat high blood pressure. Diseases associated with ACE include Hemorrhage, Intracerebral and Microvascular Complications Of Diabetes 3. Among ACE related pathways are A-beta Plaque Formation and APP Metabolism and Agents Acting on the Renin-Angiotensin System Pathway, Pharmacodynamics. The Assay Genie Human DCP / Des-gamma carboxyprothrombin ELISA is a highly sensitive assay for the quantitative measurement of DCP / Des-gamma carboxyprothrombin in serum, blood, plasma, cell culture supernatant and tissue samples.
Product Name:
Human DCP / Des-gamma carboxyprothrombin ELISA Kit
Product Code:
HUFI01807
Size:
96 Assays
Alias:
DCP, PIVKA-2
Detection method:
Sandwich ELISA, Double Antibody
Application:
This immunoassay kit allows for the in vitro quantitative determination of Human DCP concentrations in serum plasma and other biological fluids.
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human DCP and the recovery rates were calculated by comparing the measured value to the expected amount of Human DCP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
88-101
95
UFH plasma(n=5)
91-102
98
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human DCP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
85-94%
93-102%
EDTA plasma(n=5)
82-101%
82-98%
91-100%
UFH plasma(n=5)
80-100%
84-100%
81-100%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
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Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Ref.12
UniProt Protein Details:
Catalytic activity: Selective cleavage of Arg--Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
