Human DBR1 Recombinant Protein (RPPB3331)

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SKU:
RPPB3331
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UK59

Description

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Product Name:Human DBR1 Recombinant Protein
Product Code:RPPB3331
Size:10µg
Species:Human
Target:DBR1
Synonyms:Debranching RNA Lariats 1, Lariat Debranching Enzyme, Debranching Enzyme Homolog 1, RNA Lariat Debranching Enzyme, EC 3.1.-.-.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The DBR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA

DBR1 is an RNA lariat debranching enzyme which hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of eliminated lariat intron RNA, transforming them to linear molecules which are then degraded. Additionally, DBR1 takes part in retroviral replication.

DBR1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (228-544) and having a molecular mass of 38kDa (molecular size on SDS-PAGE will appear higher).DBR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DBR1: Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via a RNA lariat intermediate in cDNA synthesis. Belongs to the lariat debranching enzyme family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.1.-.-; RNA processing; RNA splicingChromosomal Location of Human Ortholog: 3q22.3Cellular Component: nucleusMolecular Function: RNA lariat debranching enzyme activityBiological Process: nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011]
UniProt Code:Q9UK59
NCBI GenInfo Identifier:56549113
NCBI Gene ID:51163
NCBI Accession:NP_057300.2
UniProt Secondary Accession:Q9UK59,Q96GH0, Q9NXQ6
UniProt Related Accession:Q9UK59
Molecular Weight:35,022 Da
NCBI Full Name:lariat debranching enzyme
NCBI Synonym Full Names:debranching RNA lariats 1
NCBI Official Symbol:DBR1��
NCBI Official Synonym Symbols:
NCBI Protein Information:lariat debranching enzyme
UniProt Protein Name:Lariat debranching enzyme
UniProt Synonym Protein Names:
Protein Family:Lariat debranching enzyme
UniProt Gene Name:DBR1��
UniProt Entry Name:DBR1_HUMAN
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