Enzymes Recombinant Proteins

Human DARS Recombinant Protein (RPPB1554)

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SKU:
RPPB1554
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P14868
Research Area:
Enzymes

Description

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Product Name:Human DARS Recombinant Protein
Product Code:RPPB1554
Size:20µg
Species:Human
Target:DARS
Synonyms:Aspartyl-tRNA synthetase, Cell proliferation-inducing gene 40 protein, AspRS, aspartate tRNA ligase 1 cytoplasmic, EC 6.1.1.12.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DARS solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 150mM Nacl, 1mM DTT and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT P

DARS uses a 2 step reaction to catalyze the specific attachment of an amino acid to its cognate tRNA: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

DARS Recombinant produced in E. coli is a single polypeptide chain containing 521 amino acids (1-501) and having a molecular mass of 59.3kDa.DARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:Translation; EC 6.1.1.12; Ligase

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function:protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity

Biological Process: tRNA aminoacylation for protein translation; translation; protein complex assembly; gene expression; aspartyl-tRNA aminoacylation

Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity

NCBI Summary:This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
UniProt Code:P14868
NCBI GenInfo Identifier:20178330
NCBI Gene ID:1615
NCBI Accession:P14868.2
UniProt Secondary Accession:P14868,Q2TNI3, Q32Q69, Q53HV4, Q53YC5, Q68CR9, Q9BW52 A8K3J2, D3DP77,
UniProt Related Accession:P14868
Molecular Weight:501
NCBI Full Name:Aspartate--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:aspartyl-tRNA synthetase
NCBI Official Symbol:DARS��
NCBI Official Synonym Symbols:HBSL; aspRS��
NCBI Protein Information:aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein
UniProt Protein Name:Aspartate--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein
UniProt Gene Name:DARS��
UniProt Entry Name:SYDC_HUMAN
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