Human Cytokine Array (60 targets) (SARB0042)
- SKU:
- SARB0042
- Product Type:
- Protein Array
- Analytes:
- Adiponectin (ACRP30)
- Analytes:
- AgRP
- Analytes:
- Angiopoietin-2
- Analytes:
- Amphiregulin
- Analytes:
- Axl
- Analytes:
- bFGF
- Analytes:
- beta-NGF
- Analytes:
- Betacellulin (BTC)
- Analytes:
- CCL28 (MEC)
- Analytes:
- CTACK (CCL27)
- Analytes:
- Dtk
- Analytes:
- EGFR
- Analytes:
- ENA-78 (CXCL5)
- Analytes:
- Fas (TNFRSF6/Apo-1)
- Analytes:
- FGF-4
- Analytes:
- FGF-9
- Analytes:
- GCSF
- Analytes:
- GITR Ligand (TNFSF18)
- Analytes:
- GITR (TNFRSF18)
- Analytes:
- GRO alpha/beta/gamma
- Analytes:
- GRO alpha (CXCL1)
- Analytes:
- HCC-4 (CCL16)
- Analytes:
- HGF
- Analytes:
- ICAM-1 (CD54)
- Analytes:
- ICAM-3 (CD50)
- Analytes:
- IGFBP-3
- Analytes:
- IGFBP-6
- Analytes:
- IGF-1 R
- Analytes:
- IL-1 R4 (ST2)
- Analytes:
- IL-1 R1
- Analytes:
- IL-11
- Analytes:
- IL-12 p40
- Analytes:
- IL-12 p70
- Analytes:
- IL-17A
- Analytes:
- IL-2 R alpha
- Analytes:
- IL-6 R
- Analytes:
- IL-8 (CXCL8)
- Analytes:
- I-TAC (CXCL11)
- Analytes:
- Lymphotactin (XCL1)
- Analytes:
- MIF
- Analytes:
- MIP-1 alpha (CCL3)
- Analytes:
- MIP-1 beta (CCL4)
- Analytes:
- MIP-3 beta (CCL19)
- Analytes:
- MSP alpha/beta
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0042 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 60 |
| Gene Symbols: | ADIPOQ, AGRP, ANGPT2, AREG, AXL, BTC, CCL16, CCL19, CCL25, CCL27|CCL28, CCL3, CCL4, CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGFR, FAS, FGF2, FGF4, FGF9, FIGF, HGF, ICAM1, ICAM3, IGF1R, IGFBP3, IGFBP6, IL11, IL12A, IL12B, IL17A, IL1R1, IL1RL1, IL2RA, IL6R, IL6ST, MIF, MST1, NGF, NTF4, OSM, PGF, PLAUR, THPO, TIMP1, TIMP2, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFSF18, TPO, TYRO3, VEGFA, XCL1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Adiponectin (ACRP30)
| AgRP
| Angiopoietin-2
| Amphiregulin
| Axl
|
bFGF
| beta-NGF
| Betacellulin (BTC)
| CCL28 (MEC)
| CTACK (CCL27)
|
Dtk
| EGFR
| ENA-78 (CXCL5)
| Fas (TNFRSF6/Apo-1)
| FGF-4
|
FGF-9
| GCSF
| GITR Ligand (TNFSF18)
| GITR (TNFRSF18)
| GRO alpha/beta/gamma
|
GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| ICAM-1 (CD54)
| ICAM-3 (CD50)
|
IGFBP-3
| IGFBP-6
| IGF-1 R
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-11
| IL-12 p40
| IL-12 p70
| IL-17A
| IL-2 R alpha
|
IL-6 R
| IL-8 (CXCL8)
| I-TAC (CXCL11)
| Lymphotactin (XCL1)
| MIF
|
MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-3 beta (CCL19)
| MSP alpha/beta
| NT-4
|
Osteoprotegerin (TNFRSF11B)
| Oncostatin M
| PLGF
| gp130
| TNF RII (TNFRSF1B)
|
TNF RI (TNFRSF1A)
| TECK (CCL25)
| TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
|
TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VEGF-A
| VEGF-D
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C7 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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