Human Cytokine Array (60 targets) (SARB0042)

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SKU:
SARB0042

Description

system_update_altDatasheet
Product Sku:SARB0042
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:60
Gene Symbols:ADIPOQ, AGRP, ANGPT2, AREG, AXL, BTC, CCL16, CCL19, CCL25, CCL27|CCL28, CCL3, CCL4, CSF3, CXCL1, CXCL11, CXCL2, CXCL3, CXCL5, CXCL8, EGFR, FAS, FGF2, FGF4, FGF9, FIGF, HGF, ICAM1, ICAM3, IGF1R, IGFBP3, IGFBP6, IL11, IL12A, IL12B, IL17A, IL1R1, IL1RL1, IL2RA, IL6R, IL6ST, MIF, MST1, NGF, NTF4, OSM, PGF, PLAUR, THPO, TIMP1, TIMP2, TNFRSF10C, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF1A, TNFRSF1B, TNFSF18, TPO, TYRO3, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Adiponectin (ACRP30)
  • Gene Symbol: ADIPOQ
  • Gene ID: 9370
  • Accession #: Q15848
AgRP
  • Gene Symbol: AgRP
  • Gene ID: 181
  • Accession #: O00253
Angiopoietin-2
  • Gene Symbol: ANGPT2
  • Gene ID: 285
  • Accession #: O15123
Amphiregulin
  • Gene Symbol: AREG
  • Gene ID: 374
  • Accession #: P15514
Axl
  • Gene Symbol: Axl
  • Gene ID: 558
  • Accession #: P30530
bFGF
  • Gene Symbol: FGF2
  • Gene ID: 2247
  • Accession #: P09038
beta-NGF
  • Gene Symbol: NGF
  • Gene ID: 4803
  • Accession #: P01138
Betacellulin (BTC)
  • Gene Symbol: BTC
  • Gene ID: 685
  • Accession #: P35070
CCL28 (MEC)
  • Gene Symbol: CCL28
  • Gene ID: 56477
  • Accession #: Q9NRJ3
CTACK (CCL27)
  • Gene Symbol: CCL27
  • Gene ID: 10850
  • Accession #: Q9Y4X3
Dtk
  • Gene Symbol: TYRO3
  • Gene ID: 7301
  • Accession #: Q06418
EGFR
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
ENA-78 (CXCL5)
  • Gene Symbol: CXCL5
  • Gene ID: 6374
  • Accession #: P42830
Fas (TNFRSF6/Apo-1)
  • Gene Symbol: Fas
  • Gene ID: 355
  • Accession #: P25445
FGF-4
  • Gene Symbol: FGF4
  • Gene ID: 2249
  • Accession #: P08620
FGF-9
  • Gene Symbol: FGF9
  • Gene ID: 2254
  • Accession #: P31371
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
GITR Ligand (TNFSF18)
  • Gene Symbol: TNFSF18
  • Gene ID: 8995
  • Accession #: Q9UNG2
GITR (TNFRSF18)
  • Gene Symbol: TNFRSF18
  • Gene ID: 8784
  • Accession #: Q9Y5U5
GRO alpha/beta/gamma
  • Gene Symbol: CXCL1/CXCL2/CXCL3
  • Gene ID: 2919/2920/2921
  • Accession #: P09341/P19875/P19876
GRO alpha (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: 2919
  • Accession #: P09341
HCC-4 (CCL16)
  • Gene Symbol: CCL16
  • Gene ID: 6360
  • Accession #: O15467
HGF
  • Gene Symbol: HGF
  • Gene ID: 3082
  • Accession #: P14210
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
ICAM-3 (CD50)
  • Gene Symbol: ICAM3
  • Gene ID: 3385
  • Accession #: P32942
IGFBP-3
  • Gene Symbol: IGFBP3
  • Gene ID: 3486
  • Accession #: P17936
IGFBP-6
  • Gene Symbol: IGFBP6
  • Gene ID: 3489
  • Accession #: P24592
IGF-1 R
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
IL-1 R4 (ST2)
  • Gene Symbol: IL1RL1
  • Gene ID: 9173
  • Accession #: Q01638
IL-1 R1
  • Gene Symbol: IL1R1
  • Gene ID: 3554
  • Accession #: P14778
IL-11
  • Gene Symbol: IL11
  • Gene ID: 3589
  • Accession #: P20809
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
  • Gene Symbol: IL2RA
  • Gene ID: 3559
  • Accession #: P01589
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
IL-8 (CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
I-TAC (CXCL11)
  • Gene Symbol: CXCL11
  • Gene ID: 6373
  • Accession #: O14625
Lymphotactin (XCL1)
  • Gene Symbol: XCL1
  • Gene ID: 6375
  • Accession #: P47992
MIF
  • Gene Symbol: MIF
  • Gene ID: 4282
  • Accession #: P14174
MIP-1 alpha (CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
MIP-1 beta (CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
MIP-3 beta (CCL19)
  • Gene Symbol: CCL19
  • Gene ID: 6363
  • Accession #: Q99731
MSP alpha/beta
  • Gene Symbol: MST1
  • Gene ID: 4485
  • Accession #: P26927
NT-4
  • Gene Symbol: NTF4
  • Gene ID: 4909
  • Accession #: P34130
Osteoprotegerin (TNFRSF11B)
  • Gene Symbol: TNFRSF11B
  • Gene ID: 4982
  • Accession #: O00300
Oncostatin M
  • Gene Symbol: OSM
  • Gene ID: 5008
  • Accession #: P13725
PLGF
  • Gene Symbol: PGF
  • Gene ID: 5228
  • Accession #: P49763
gp130
  • Gene Symbol: Il6st
  • Gene ID: 3572
  • Accession #: P40189
TNF RII (TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: 7133
  • Accession #: P20333
TNF RI (TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
TECK (CCL25)
  • Gene Symbol: CCL25
  • Gene ID: 6370
  • Accession #: O15444
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
Thrombopoietin (TPO)
  • Gene Symbol: THPO
  • Gene ID: 7066
  • Accession #: P40225
TRAIL R3 (TNFRSF10C)
  • Gene Symbol: TNFRSF10C
  • Gene ID: 8794
  • Accession #: O14798
TRAIL R4 (TNFRSF10D)
  • Gene Symbol: TNFRSF10D
  • Gene ID: 8793
  • Accession #: Q9UBN6
uPAR
  • Gene Symbol: PLAUR
  • Gene ID: 5329
  • Accession #: Q03405
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
VEGF-D
  • Gene Symbol: FIGF
  • Gene ID: 2277
  • Accession #: O43915
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Adiponectin (ACRP30)
Analytes:
AgRP
Analytes:
Angiopoietin-2
Analytes:
Amphiregulin
Analytes:
Axl
Analytes:
bFGF
Analytes:
beta-NGF
Analytes:
Betacellulin (BTC)
Analytes:
CCL28 (MEC)
Analytes:
CTACK (CCL27)
Analytes:
Dtk
Analytes:
EGFR
Analytes:
ENA-78 (CXCL5)
Analytes:
Fas (TNFRSF6/Apo-1)
Analytes:
FGF-4
Analytes:
FGF-9
Analytes:
GCSF
Analytes:
GITR Ligand (TNFSF18)
Analytes:
GITR (TNFRSF18)
Analytes:
GRO alpha/beta/gamma
Analytes:
GRO alpha (CXCL1)
Analytes:
HCC-4 (CCL16)
Analytes:
HGF
Analytes:
ICAM-1 (CD54)
Analytes:
ICAM-3 (CD50)
Analytes:
IGFBP-3
Analytes:
IGFBP-6
Analytes:
IGF-1 R
Analytes:
IL-1 R4 (ST2)
Analytes:
IL-1 R1
Analytes:
IL-11
Analytes:
IL-12 p40
Analytes:
IL-12 p70
Analytes:
IL-17A
Analytes:
IL-2 R alpha
Analytes:
IL-6 R
Analytes:
IL-8 (CXCL8)
Analytes:
I-TAC (CXCL11)
Analytes:
Lymphotactin (XCL1)
Analytes:
MIF
Analytes:
MIP-1 alpha (CCL3)
Analytes:
MIP-1 beta (CCL4)
Analytes:
MIP-3 beta (CCL19)
Analytes:
MSP alpha/beta
Reactivity:
Human
Applications:
Multiplex Array
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