Human Cytokine Array (60 targets) (SARB0041)

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SKU:
SARB0041
Product Type:
Protein Array
Analytes:
Angiogenin
Analytes:
BDNF
Analytes:
BLC (CXCL13)
Analytes:
BMP-4
Analytes:
BMP-6
Analytes:
Ck beta 8-1 (CCL23)
Analytes:
CNTF
Analytes:
EGF
Analytes:
Eotaxin-1 (CCL11)
Analytes:
Eotaxin-2 (MPIF-2/CCL24)
Analytes:
Eotaxin-3 (CCL26)
Analytes:
FGF-6
Analytes:
FGF-7 (KGF)
Analytes:
Flt-3 Ligand
Analytes:
Fractalkine (CX3CL1)
Analytes:
GCP-2 (CXCL6)
Analytes:
GDNF
Analytes:
GM-CSF
Analytes:
I-309 (TCA-3/CCL1)
Analytes:
IFN-gamma
Analytes:
IGFBP-1
Analytes:
IGFBP-2
Analytes:
IGFBP-4
Analytes:
IGF-1
Analytes:
IL-10
Analytes:
IL-13
Analytes:
IL-15
Analytes:
IL-16
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 ra (IL-1 F3)
Analytes:
IL-2
Analytes:
IL-3
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-6
Analytes:
IL-7
Analytes:
Leptin
Analytes:
Light (TNFSF14)
Analytes:
MCP-1 (CCL2)
Analytes:
MCP-2 (CCL8)
Analytes:
MCP-3 (MARC/CCL7)
Analytes:
MCP-4 (CCL13)
Analytes:
M-CSF
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0041
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:60
Gene Symbols:ANG, BDNF, BMP4, BMP6, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18|CCL2, CCL20, CCL22, CCL23, CCL24, CCL26, CCL5, CCL7, CCL8, CNTF, CSF1, CSF2, CX3CL1, CXCL12, CXCL13, CXCL6, CXCL9, EGF, FGF6, FGF7, FLT3LG, GDNF, IFNG, IGF1, IGFBP1, IGFBP2, IGFBP4, IL10, IL13, IL15, IL16, IL1A, IL1B, IL1RN, IL2, IL3, IL4, IL5, IL6, IL7, KITLG, LEP, LTA, NTF3, PDGFB, PPBP, TGFB1, TGFB3, TNF, TNFSF14
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Angiogenin
  • Gene Symbol: ANG
  • Gene ID: 283
  • Accession #: P03950
BDNF
  • Gene Symbol: BDNF
  • Gene ID: 627
  • Accession #: P23560
BLC (CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
BMP-4
  • Gene Symbol: BMP4
  • Gene ID: 652
  • Accession #: P12644
BMP-6
  • Gene Symbol: BMP6
  • Gene ID: 654
  • Accession #: P22004
Ck beta 8-1 (CCL23)
  • Gene Symbol: CCL23
  • Gene ID: 6368
  • Accession #: P55773
CNTF
  • Gene Symbol: CNTF
  • Gene ID: 1270
  • Accession #: P26441
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #: P01133
Eotaxin-1 (CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
Eotaxin-2 (MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
Eotaxin-3 (CCL26)
  • Gene Symbol: CCL26
  • Gene ID: 10344
  • Accession #: Q9Y258
FGF-6
  • Gene Symbol: FGF6
  • Gene ID: 2251
  • Accession #: P10767
FGF-7 (KGF)
  • Gene Symbol: FGF7
  • Gene ID: 2252
  • Accession #: P21781
Flt-3 Ligand
  • Gene Symbol: FLT3LG
  • Gene ID: 2323
  • Accession #: P49771
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: 6376
  • Accession #: P78423
GCP-2 (CXCL6)
  • Gene Symbol: CXCL6
  • Gene ID: 6372
  • Accession #: P80162
GDNF
  • Gene Symbol: GDNF
  • Gene ID: 2668
  • Accession #: P39905
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
I-309 (TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
IGFBP-1
  • Gene Symbol: IGFBP1
  • Gene ID: 3484
  • Accession #: P08833
IGFBP-2
  • Gene Symbol: IGFBP2
  • Gene ID: 3485
  • Accession #: P18065
IGFBP-4
  • Gene Symbol: IGFBP4
  • Gene ID: 3487
  • Accession #: P22692
IGF-1
  • Gene Symbol: IGF1
  • Gene ID: 3479
  • Accession #: P05019
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
IL-1 ra (IL-1 F3)
  • Gene Symbol: IL1RN
  • Gene ID: 3557
  • Accession #: P18510
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
IL-3
  • Gene Symbol: IL3
  • Gene ID: 3562
  • Accession #: P08700
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
Leptin
  • Gene Symbol: LEP
  • Gene ID: 3952
  • Accession #: P41159
Light (TNFSF14)
  • Gene Symbol: TNFSF14
  • Gene ID: 8740
  • Accession #: O43557
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
MCP-2 (CCL8)
  • Gene Symbol: CCL8
  • Gene ID: 6355
  • Accession #: P80075
MCP-3 (MARC/CCL7)
  • Gene Symbol: CCL7
  • Gene ID: 6354
  • Accession #: P80098
MCP-4 (CCL13)
  • Gene Symbol: CCL13
  • Gene ID: 6357
  • Accession #: Q99616
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
MDC (CCL22)
  • Gene Symbol: CCL22
  • Gene ID: 6367
  • Accession #: O00626
MIG (CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
MIP-1 delta (CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: 6364
  • Accession #: P78556
NAP-2 (PPBP/CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: 5473
  • Accession #: P02775
NT-3
  • Gene Symbol: NTF3
  • Gene ID: 4908
  • Accession #: P20783
PARC (CCL18)
  • Gene Symbol: CCL18
  • Gene ID: 6362
  • Accession #: P55774
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
RANTES (CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
SCF
  • Gene Symbol: KITLG
  • Gene ID: 4254
  • Accession #: P21583
SDF-1 alpha (CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Gene ID: 6387
  • Accession #: P48061
TARC (CCL17)
  • Gene Symbol: CCL17
  • Gene ID: 6361
  • Accession #: Q92583
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
TNF beta (TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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