Human Cytokine Array (51 targets) (SARB0044)

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SKU:
SARB0044

Description

system_update_altDatasheet
Product Sku:SARB0044
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:51
Gene Symbols:ADAM17, AGER, BCAM, CD40, CFD, CGA, EDA2R, FCGR2B, FST, FSTL3|FTH1, FURIN, GDF15, GH1, HAVCR1, IFNL1, IFNL2, IL10RA, IL22, IL31, INS, KLK3, LGALS7, LHB, LYVE1, MICA, MICB, MMP10, MMP2, MMP7, MMP8, NCAM1, NID1, NRCAM, NRG1, PF4, PI3, PRSS27, RETN, SAA1, SCARB2, SERPINE1, SIGLEC9, SPP1, THRB, TNFRSF10B, TNFRSF11A, TNFRSF8, TREM1, TSLP, VCAM1, VEGFC
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Adipsin (Complement Factor D)
  • Gene Symbol: CFD
  • Gene ID: 1675
  • Accession #: P00746
BCAM
  • Gene Symbol: BCAM
  • Gene ID: 4059
  • Accession #: P50895
CD30 (TNFRSF8)
  • Gene Symbol: CD30
  • Gene ID: 943
  • Accession #: P28908
CD40 (TNFRSF5)
  • Gene Symbol: CD40
  • Gene ID: 958
  • Accession #: P25942
Fc gamma RIIB/C (CD32B/C)
  • Gene Symbol: FCGR2B
  • Gene ID: 2213
  • Accession #: P31994
Ferritin
  • Gene Symbol: FTH1
  • Gene ID: 2495
  • Accession #: P02794
FLRG
  • Gene Symbol: FSTL3
  • Gene ID: 10272
  • Accession #: O95633
Follistatin
  • Gene Symbol: FST
  • Gene ID: 10468
  • Accession #: P19883
Furin
  • Gene Symbol: Furin
  • Gene ID: 5045
  • Accession #: P09958
Galectin-7
  • Gene Symbol: LGALS7
  • Gene ID: 3963
  • Accession #: P47929
GDF-15
  • Gene Symbol: GDF15
  • Gene ID: 9518
  • Accession #: Q99988
Growth Hormone
  • Gene Symbol: GH1
  • Gene ID: 2688
  • Accession #: P01241
IL-10 R alpha
  • Gene Symbol: IL10RA
  • Gene ID: 3587
  • Accession #: Q13651
IL-22
  • Gene Symbol: IL22
  • Gene ID: 50616
  • Accession #: Q9GZX6
IL-28A (IFN-lambda 2)
  • Gene Symbol: IFNL2
  • Gene ID: 282616
  • Accession #: Q8IZJ0
IL-29 (IFN-lambda 1)
  • Gene Symbol: IFNL1
  • Gene ID: 282618
  • Accession #: Q8IU54
IL-31
  • Gene Symbol: IL31
  • Gene ID: 386653
  • Accession #: Q6EBC2
Insulin
  • Gene Symbol: INS
  • Gene ID: 3630
  • Accession #: P01308
Luteinizing hormone
  • Gene Symbol: LHB
  • Gene ID: 3972
  • Accession #: P01229
LIMPII
  • Gene Symbol: SCARB2
  • Gene ID: 950
  • Accession #: Q14108
LYVE-1
  • Gene Symbol: LYVE1
  • Gene ID: 10894
  • Accession #: Q9Y5Y7
Marapsin
  • Gene Symbol: PRSS27
  • Gene ID: 83886
  • Accession #: Q9BQR3
MICA
  • Gene Symbol: MICA
  • Gene ID: 100507436
  • Accession #: Q29983
MICB
  • Gene Symbol: MICB
  • Gene ID: 4277
  • Accession #: Q29980
MMP-2
  • Gene Symbol: MMP2
  • Gene ID: 4313
  • Accession #: P08253
MMP-7
  • Gene Symbol: MMP7
  • Gene ID: 4316
  • Accession #: P09237
MMP-8
  • Gene Symbol: MMP8
  • Gene ID: 4317
  • Accession #: P22894
MMP-10
  • Gene Symbol: MMP10
  • Gene ID: 4319
  • Accession #: P09238
NCAM-1 (CD56)
  • Gene Symbol: NCAM1
  • Gene ID: 4684
  • Accession #: P13591
Nidogen-1
  • Gene Symbol: NID1
  • Gene ID: 4811
  • Accession #: P14543
NrCAM
  • Gene Symbol: NrCAM
  • Gene ID: 4897
  • Accession #: Q92823
NRG1-beta 1 (HRG1-beta 1)
  • Gene Symbol: NRG1
  • Gene ID: 3084
  • Accession #: Q02297
Osteopontin (SPP1)
  • Gene Symbol: SPP1
  • Gene ID: 6696
  • Accession #: P10451
PAI-1
  • Gene Symbol: SERPINE1
  • Gene ID: 5054
  • Accession #: P05121
Platelet factor 4 (CXCL4)
  • Gene Symbol: PF4 CXCL4 SCYB4
  • Gene ID: 5196
  • Accession #: P02776
PSA-total
  • Gene Symbol: KLK3
  • Gene ID: 354
  • Accession #: P07288
RAGE
  • Gene Symbol: MOK
  • Gene ID: 5891
  • Accession #: Q9UQ07
RANK (TNFRSF11A)
  • Gene Symbol: TNFRSF11A
  • Gene ID: 8792
  • Accession #: Q9Y6Q6
Resistin
  • Gene Symbol: RETN
  • Gene ID: 56729
  • Accession #: Q9HD89
SAA (Serum Amyloid A)
  • Gene Symbol: SAA1
  • Gene ID: 6288
  • Accession #: P0DJI8
Siglec-9
  • Gene Symbol: SIGLEC9
  • Gene ID: 27180
  • Accession #: Q9Y336
TACE
  • Gene Symbol: TACE
  • Gene ID: N/A
  • Accession #: N/A
TIM-1 (KIM-1)
  • Gene Symbol: HAVCR1
  • Gene ID: 26762
  • Accession #: Q96D42
TRAIL R2 (TNFRSF10B/DR5)
  • Gene Symbol: TNFRSF10B
  • Gene ID: 8795
  • Accession #: O14763
Trappin-2
  • Gene Symbol: PI3
  • Gene ID: 5266
  • Accession #: P19957
TREM-1
  • Gene Symbol: TREM1
  • Gene ID: 54210
  • Accession #: Q9NP99
TSH
  • Gene Symbol: CGA; TSHB
  • Gene ID: N/A
  • Accession #: N/A
TSLP
  • Gene Symbol: TSLP
  • Gene ID: 85480
  • Accession #: Q969D9
VCAM-1 (CD106)
  • Gene Symbol: VCAM1
  • Gene ID: 7412
  • Accession #: P19320
VEGF-C
  • Gene Symbol: VEGFC
  • Gene ID: 7424
  • Accession #: P49767
XEDAR
  • Gene Symbol: EDA2R
  • Gene ID: 60401
  • Accession #: Q9HAV5
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C9 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Adipsin (Complement Factor D)
Analytes:
BCAM
Analytes:
CD30 (TNFRSF8)
Analytes:
CD40 (TNFRSF5)
Analytes:
Fc gamma RIIB/C (CD32B/C)
Analytes:
Ferritin
Analytes:
FLRG
Analytes:
Follistatin
Analytes:
Furin
Analytes:
Galectin-7
Analytes:
GDF-15
Analytes:
Growth Hormone
Analytes:
IL-10 R alpha
Analytes:
IL-22
Analytes:
IL-28A (IFN-lambda 2)
Analytes:
IL-29 (IFN-lambda 1)
Analytes:
IL-31
Analytes:
Insulin
Analytes:
Luteinizing hormone
Analytes:
LIMPII
Analytes:
LYVE-1
Analytes:
Marapsin
Analytes:
MICA
Analytes:
MICB
Analytes:
MMP-2
Analytes:
MMP-7
Analytes:
MMP-8
Analytes:
MMP-10
Analytes:
NCAM-1 (CD56)
Analytes:
Nidogen-1
Analytes:
NrCAM
Analytes:
NRG1-beta 1 (HRG1-beta 1)
Analytes:
Osteopontin (SPP1)
Analytes:
PAI-1
Analytes:
Platelet factor 4 (CXCL4)
Analytes:
PSA-total
Analytes:
RAGE
Analytes:
RANK (TNFRSF11A)
Analytes:
Resistin
Analytes:
SAA (Serum Amyloid A)
Analytes:
Siglec-9
Analytes:
TACE
Analytes:
TIM-1 (KIM-1)
Analytes:
TRAIL R2 (TNFRSF10B/DR5)
Reactivity:
Human
Applications:
Multiplex Array
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