Human Cytokine Array (40 targets) (SARB0033)

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SKU:
SARB0033

Description

system_update_altDatasheet
Product Sku:SARB0033
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:40
Gene Symbols:ADAM9, BMP2, C5, CD200, CD244, CD97, CTSL, FABP2, FAP, FGF19|FRZB, GDF2, GPNMB, IFNAR2, IGF2R, IL1RL2, IL24, IL33, KLK14, LGALS3, LGMN, MBL2, MET, MME, NOTCH1, NOV, OLR1, PDCD1, PGLYRP1, RARRES2, SERPINA4, TF, THBD, TLR2, TNFRSF6B, TNFSF13, WIF1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
2B4 (CD244)
  • Gene Symbol: CD244
  • Gene ID: 51744
  • Accession #: Q9BZW8
ADAM-9
  • Gene Symbol: ADAM9
  • Gene ID: 8754
  • Accession #: Q13443
APRIL
  • Gene Symbol: TNFSF13
  • Gene ID: 8741
  • Accession #: O75888
BMP-2
  • Gene Symbol: BMP2
  • Gene ID: 650
  • Accession #: P12643
BMP-9
  • Gene Symbol: GDF2
  • Gene ID: 2658
  • Accession #: Q9UK05
Cathepsin L
  • Gene Symbol: CTSL
  • Gene ID: 1514
  • Accession #: P07711
CD200
  • Gene Symbol: CD200
  • Gene ID: 4345
  • Accession #: P41217
CD97
  • Gene Symbol: CD97
  • Gene ID: 976
  • Accession #: P48960
Chemerin
  • Gene Symbol: RARRES2
  • Gene ID: 5919
  • Accession #: Q99969
C5a
  • Gene Symbol: C5
  • Gene ID: 727
  • Accession #: P01031
DcR3
  • Gene Symbol: TNFRSF6B
  • Gene ID: 8771
  • Accession #: O95407
FABP2
  • Gene Symbol: FABP2
  • Gene ID: 2169
  • Accession #: P12104
FAP
  • Gene Symbol: FAP
  • Gene ID: 2191
  • Accession #: Q12884
FGF-19
  • Gene Symbol: FGF19
  • Gene ID: 9965
  • Accession #: O95750
Galectin-3
  • Gene Symbol: LGALS3
  • Gene ID: 3958
  • Accession #: P17931
HGFR
  • Gene Symbol: MET
  • Gene ID: 4233
  • Accession #: P08581
IFN-alpha / beta R2
  • Gene Symbol: IFNAR2
  • Gene ID: 3455
  • Accession #: P48551
IGF-2 R
  • Gene Symbol: IGF2R
  • Gene ID: 3482
  • Accession #: P11717
IL-1 R6 (IL-1 Rrp2)
  • Gene Symbol: IL1RL2
  • Gene ID: 8808
  • Accession #: Q9HB29
IL-24
  • Gene Symbol: IL24
  • Gene ID: 11009
  • Accession #: Q13007
IL-33 (IL-1 F11)
  • Gene Symbol: IL33
  • Gene ID: 90865
  • Accession #: O95760
Kallikrein 14
  • Gene Symbol: KLK14
  • Gene ID: 43847
  • Accession #: Q9P0G3
Legumain
  • Gene Symbol: LGMN
  • Gene ID: 5641
  • Accession #: Q99538
LOX-1
  • Gene Symbol: OLR1
  • Gene ID: 4973
  • Accession #: P78380
MBL
  • Gene Symbol: MBL
  • Gene ID: N/A
  • Accession #: N/A
Neprilysin
  • Gene Symbol: MME
  • Gene ID: 4311
  • Accession #: P08473
Notch-1
  • Gene Symbol: NOTCH1
  • Gene ID: 4851
  • Accession #: P46531
NOV (CCN3)
  • Gene Symbol: NOV
  • Gene ID: 4856
  • Accession #: P48745
Osteoactivin (GPNMB)
  • Gene Symbol: GPNMB
  • Gene ID: 10457
  • Accession #: Q14956
PD-1
  • Gene Symbol: PDCD1
  • Gene ID: 5133
  • Accession #: Q15116
PGRPs
  • Gene Symbol: PGLYRP1
  • Gene ID: 8993
  • Accession #: O75594
SERPINA4
  • Gene Symbol: SERPINA4
  • Gene ID: 5267
  • Accession #: P29622
sFRP-3
  • Gene Symbol: FRZB
  • Gene ID: 2487
  • Accession #: Q92765
Thrombomodulin
  • Gene Symbol: THBD
  • Gene ID: 7056
  • Accession #: P07204
TLR2
  • Gene Symbol: TLR2
  • Gene ID: 7097
  • Accession #: O60603
Transferrin
  • Gene Symbol: TF
  • Gene ID: 7018
  • Accession #: P02787
WIF-1
  • Gene Symbol: WIF1
  • Gene ID: 11197
  • Accession #: Q9Y5W5
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Cytokine Antibody Array C11 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
2B4 (CD244)
Analytes:
ADAM-9
Analytes:
APRIL
Analytes:
BMP-2
Analytes:
BMP-9
Analytes:
Cathepsin L
Analytes:
CD200
Analytes:
CD97
Analytes:
Chemerin
Analytes:
C5a
Analytes:
DcR3
Analytes:
FABP2
Analytes:
FAP
Analytes:
FGF-19
Analytes:
Galectin-3
Analytes:
HGFR
Analytes:
IFN-alpha / beta R2
Analytes:
IGF-2 R
Analytes:
IL-1 R6 (IL-1 Rrp2)
Analytes:
IL-24
Analytes:
IL-33 (IL-1 F11)
Analytes:
Kallikrein 14
Analytes:
Legumain
Analytes:
LOX-1
Analytes:
MBL
Analytes:
Neprilysin
Analytes:
Notch-1
Analytes:
NOV (CCN3)
Analytes:
Osteoactivin (GPNMB)
Analytes:
PD-1
Analytes:
PGRPs
Analytes:
SERPINA4
Analytes:
sFRP-3
Analytes:
Thrombomodulin
Analytes:
TLR2
Analytes:
Transferrin
Analytes:
WIF-1
Reactivity:
Human
Applications:
Multiplex Array
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