Human Cytokine Array (40 targets) (SARB0033)
- SKU:
- SARB0033
- Product Type:
- Protein Array
- Analytes:
- 2B4 (CD244)
- Analytes:
- ADAM-9
- Analytes:
- APRIL
- Analytes:
- BMP-2
- Analytes:
- BMP-9
- Analytes:
- Cathepsin L
- Analytes:
- CD200
- Analytes:
- CD97
- Analytes:
- Chemerin
- Analytes:
- C5a
- Analytes:
- DcR3
- Analytes:
- FABP2
- Analytes:
- FAP
- Analytes:
- FGF-19
- Analytes:
- Galectin-3
- Analytes:
- HGFR
- Analytes:
- IFN-alpha / beta R2
- Analytes:
- IGF-2 R
- Analytes:
- IL-1 R6 (IL-1 Rrp2)
- Analytes:
- IL-24
- Analytes:
- IL-33 (IL-1 F11)
- Analytes:
- Kallikrein 14
- Analytes:
- Legumain
- Analytes:
- LOX-1
- Analytes:
- MBL
- Analytes:
- Neprilysin
- Analytes:
- Notch-1
- Analytes:
- NOV (CCN3)
- Analytes:
- Osteoactivin (GPNMB)
- Analytes:
- PD-1
- Analytes:
- PGRPs
- Analytes:
- SERPINA4
- Analytes:
- sFRP-3
- Analytes:
- Thrombomodulin
- Analytes:
- TLR2
- Analytes:
- Transferrin
- Analytes:
- WIF-1
- Reactivity:
- Human
- Applications:
- Multiplex Array
Description
system_update_altDatasheet
| Product Sku: | SARB0033 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 40 |
| Gene Symbols: | ADAM9, BMP2, C5, CD200, CD244, CD97, CTSL, FABP2, FAP, FGF19|FRZB, GDF2, GPNMB, IFNAR2, IGF2R, IL1RL2, IL24, IL33, KLK14, LGALS3, LGMN, MBL2, MET, MME, NOTCH1, NOV, OLR1, PDCD1, PGLYRP1, RARRES2, SERPINA4, TF, THBD, TLR2, TNFRSF6B, TNFSF13, WIF1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
2B4 (CD244)
| ADAM-9
| APRIL
| BMP-2
| BMP-9
|
Cathepsin L
| CD200
| CD97
| Chemerin
| C5a
|
DcR3
| FABP2
| FAP
| FGF-19
| Galectin-3
|
HGFR
| IFN-alpha / beta R2
| IGF-2 R
| IL-1 R6 (IL-1 Rrp2)
| IL-24
|
IL-33 (IL-1 F11)
| Kallikrein 14
| Legumain
| LOX-1
| MBL
|
Neprilysin
| Notch-1
| NOV (CCN3)
| Osteoactivin (GPNMB)
| PD-1
|
PGRPs
| SERPINA4
| sFRP-3
| Thrombomodulin
| TLR2
|
Transferrin
| WIF-1
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Cytokine Antibody Array C11 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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