Human CYP2E1 Recombinant Protein (RPPB1550)
- SKU:
- RPPB1550
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P05181
Description
| Product Name: | Human CYP2E1 Recombinant Protein |
| Product Code: | RPPB1550 |
| Size: | 20µg |
| Species: | Human |
| Target: | CYP2E1 |
| Synonyms: | Cytochrome P450, Family 2, Subfamily E, Polypeptide 1,CYP2E, Cytochrome P450, Subfamily IIE (Ethanol-Inducible), Polypeptide 1, 4-Nitrophenol2-Hydroxylase, Cytochrome P450-J, P450C2E, CYPIIE1, Flavoprotein-LinkedMonooxygenase, Microsomal Monooxygenase, Xenobiotic Monooxygenase, CytochromeP450 2E1, EC 1.14.13.n7, EC 1.14.14.1, EC 1.14.13.-, P450-J, CPE1, CytochromeP450 2E1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CYP2E1 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQRMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNESRIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWLQLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYTMDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVHEIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSDYFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS |
CYP2E1, also known as Cytochrome P450 2E1 belongs to �the cytochrome P450 mixed-function oxidasesystem which is involved in the metabolism of xenobiotics in the body. While CYP2E1is implicated in the oxidative metabolism of small range substrates, commonlysmall polar molecules, there are many significant drug interactions which are mediatedby CYP2E1.
CYP2E1 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 488 amino acids (29-493 a.a) andhaving a molecular mass of 56.2kDa.CYP2E1 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.13.n7; Lipid Metabolism - arachidonic acid; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Mitochondrial Chromosomal Location of Human Ortholog: 10q26.3 Cellular Component: endoplasmic reticulum membrane Molecular Function:arachidonic acid epoxygenase activity; enzyme binding; heme binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity Biological Process: benzene metabolic process; carbon tetrachloride metabolic process; drug metabolic process; epoxygenase P450 pathway; halogenated hydrocarbon metabolic process; heterocycle metabolic process; monoterpenoid metabolic process; steroid metabolic process; xenobiotic metabolic process |
| NCBI Summary: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05181 |
| NCBI GenInfo Identifier: | 117250 |
| NCBI Gene ID: | 1571 |
| NCBI Accession: | P05181.1 |
| UniProt Secondary Accession: | P05181,Q5VZD5, Q6NWT9, Q9UK47, |
| UniProt Related Accession: | P05181 |
| Molecular Weight: | 56,849 Da |
| NCBI Full Name: | Cytochrome P450 2E1 |
| NCBI Synonym Full Names: | cytochrome P450 family 2 subfamily E member 1 |
| NCBI Official Symbol: | CYP2E1�� |
| NCBI Official Synonym Symbols: | CPE1; CYP2E; P450-J; P450C2E�� |
| NCBI Protein Information: | cytochrome P450 2E1 |
| UniProt Protein Name: | Cytochrome P450 2E1 |
| UniProt Synonym Protein Names: | 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-J |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYP2E1�� |
| UniProt Entry Name: | CP2E1_HUMAN |
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