Enzymes Recombinant Proteins

Human Cyclophilin A Recombinant Protein (RPPB1531)

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SKU:
RPPB1531
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P62937
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human Cyclophilin A Recombinant Protein
Product Code:RPPB1531
Size:50µg
Species:Human
Target:Cyclophilin A
Synonyms:Peptidylprolyl isomerase A, CYPA, CYPH, MGC12404, MGC23397, MGC117158, PPIase A, Rotamase A, PPIA, Peptidyl-prolyl cis-trans isomerase A, EC 5.2.1.8, Cyclophilin A, Cyclosporin A-binding protein.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:1 mg/ml solution containing 20mM Tris-HCl 8.0, 20mM NaCl, 0.5mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALSTGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSMANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIADCGQLE
Biological Activity:Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37�C in Tris-HCl pH8.0 using chymotrypsin.

PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Cyclophilin-A is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. Cyclophilin-A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Cyclophilin-A Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 185 amino acids (1-165 a.a.) and having a molecular mass of 20 kDa. PPIase-A is purified by proprietary chromatographic techniques.

UniProt Protein Function:PPIA: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Interacts with HIV-1 Capsid protein. Binds cyclosporin A (CsA). CsA mediates some of its effects via an inhibitory action on PPIase. Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
UniProt Protein Details:

Protein type:Isomerase; RNA-binding; Cyclophilin; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: cytosol; extracellular region; extracellular space; focal adhesion; membrane; nucleus

Molecular Function:peptidyl-prolyl cis-trans isomerase activity; protein binding; unfolded protein binding

Biological Process: entry into host cell; leukocyte migration; positive regulation of protein secretion; positive regulation of viral genome replication; protein folding; protein peptidyl-prolyl isomerization; regulation of viral genome replication; release of virus from host; RNA-dependent DNA replication; uncoating of virus; viral envelope fusion with host membrane; viral infectious cycle; virus assembly

NCBI Summary:This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:P62937
NCBI GenInfo Identifier:51702775
NCBI Gene ID:5478
NCBI Accession:P62937.2
UniProt Secondary Accession:P62937,P05092, Q3KQW3, Q567Q0, Q6IBU5, Q96IX3, Q9BRU4 Q9BTY9, Q9UC61, A8K220,
UniProt Related Accession:P62937
Molecular Weight:11,418 Da
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase A
NCBI Synonym Full Names:peptidylprolyl isomerase A
NCBI Official Symbol:PPIA��
NCBI Official Synonym Symbols:CYPA; CYPH; HEL-S-69p��
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase A
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase A
UniProt Synonym Protein Names:Cyclophilin A; Cyclosporin A-binding protein; Rotamase A
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:PPIA��
UniProt Entry Name:PPIA_HUMAN
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system_update_altDatasheetHuman Cyclophilin A Recombinant Protein Peptidyl-prolyl cis-trans isomerase A; also known as PPIase A; Rotamase A; Cyclophilin A; Cyclosporin A-binding protein; PPIA a