Human CYB5A Recombinant Protein (RPPB3318)

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SKU:
RPPB3318
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P00167

Description

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Product Name:Human CYB5A Recombinant Protein
Product Code:RPPB3318
Size:10µg
Species:Human
Target:CYB5A
Synonyms:Cytochrome b5 isoform 1, CYB5A, CYB5, MCB5, Microsomal cytochrome b5 type A.
Source:Sf9 Insect cells
Physical Appearance:Sterile filtered colorless solution.
Formulation:CYB5A protein solution (1mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPMAEQSDE AVKYYTLEEI QKHNHSKSTW LILHHKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDAREMSK TFIIGELHPDDRPKLNKPPE TLITTIDSSS SHHHHHH

Cytochrome b5 isoform 1 or CYB5A, is part of the cytochrome b5 family which is bound to the cell membrane. CYB5A has a key role in the stearyl-CoA-desaturase activity as it reduces ferric hemoglobin to ferrous hemoglobin. Cytochrome b5 isoform 1 performs as an electron carrier for numerous� oxygenases that are imbedded in the cell membrane.�

CYB5A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 117 amino acids (1-108a.a.) and having a molecular mass of 13.3kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).CYB5A is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane

Molecular Function:cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding

Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process

Disease: Methemoglobinemia Type Iv

NCBI Summary:The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:P00167
NCBI GenInfo Identifier:117809
NCBI Gene ID:1528
NCBI Accession:P00167.2
UniProt Secondary Accession:P00167,Q6IB14, A8MV91, F8WEU4,
UniProt Related Accession:P00167
Molecular Weight:14,169 Da
NCBI Full Name:Cytochrome b5
NCBI Synonym Full Names:cytochrome b5 type A (microsomal)
NCBI Official Symbol:CYB5A��
NCBI Official Synonym Symbols:CYB5; MCB5��
NCBI Protein Information:cytochrome b5; type 1 cyt-b5
UniProt Protein Name:Cytochrome b5
UniProt Synonym Protein Names:Microsomal cytochrome b5 type A; MCB5
Protein Family:Cytochrome
UniProt Gene Name:CYB5A��
UniProt Entry Name:CYB5_HUMAN
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