Immunology Recombinant Proteins
Human CTLA4 Recombinant Protein (RPES3191)
- SKU:
- RPES3191
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Immunology
Description
Human CTLA4 Recombinant Protein
Cytotoxic Tlymphocyte 4(CTLA-4;CD152); is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence; a 126 aa extracellular domain (ECD) with one Ig-like V-type domain; a 21 aa transmembrane (TM) sequence; and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
| Product Name: | Human CTLA4 Recombinant Protein (RPES3191) |
| Product Code: | RPES3191 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4,CTLA4,CD152,Cytotoxic T-Lymphocyte-Associated Protein 4 |
| Accession: | P16410 |
| Sequence: | Lys36-Asp161 |
| Fusion tag: | C-His |
| Activity: | Immobilized Mouse B7-1-Fc(Cat: PKSM041366) at 10µg/ml(100 ?l/well) can bind Human CTLA-4-His. The ED50 of Human CTLA-4-His is 2.3 ng/ml . |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Cytotoxic CTLA4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 14.3 kDa |
| AP Mol Mass: | 20-25 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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