Human CST6 Recombinant Protein (RPPB3293)

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SKU:
RPPB3293
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15828

Description

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Product Name:Human CST6 Recombinant Protein
Product Code:RPPB3293
Size:10µg
Species:Human
Target:CST6
Synonyms:Cystatin E/M, cystatin 6, Cystatin M, Cystatin-E, Cysteine proteinase inhibitor.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The CST6 solution (0.5mg/1ml) contains 10% glycerol, 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM
Biological Activity:The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25?C.

Cystatin E/M or CST6 is part of the cystatin type 2 family. Part of the cystatin type 2 family members can act as cysteine protease inhibitors, whereas cystatin E/M regulates cathepsin B inhibitors and not cathepsin C. cystatin E/M is a protein the when secreted, has an effect on osteogenesis and bone resorption, insulin regulation, response to systemic inflammation & hepatocyte growth factor receptors.

CST6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 142 amino acids (29-149 a.a.) and having a molecular mass of 15.9kDa.CST6 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.
UniProt Protein Details:

Protein type:Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cornified envelope

Molecular Function:cysteine protease inhibitor activity

Biological Process: epidermis development; anatomical structure morphogenesis

NCBI Summary:The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype. [provided by RefSeq, Jul 2008]
UniProt Code:Q15828
NCBI GenInfo Identifier:4503113
NCBI Gene ID:1474
NCBI Accession:NP_001314.1
UniProt Secondary Accession:Q15828,Q540N7,
UniProt Related Accession:Q15828
Molecular Weight:16,511 Da
NCBI Full Name:cystatin-M
NCBI Synonym Full Names:cystatin E/M
NCBI Official Symbol:CST6��
NCBI Protein Information:cystatin-M; cystatin 6; cystatin M; cystatin-6; cystatin-E; cystatin M/E; cysteine proteinase inhibitor
UniProt Protein Name:Cystatin-M
UniProt Synonym Protein Names:Cystatin-6; Cystatin-E
Protein Family:Cystatin
UniProt Gene Name:CST6��
UniProt Entry Name:CYTM_HUMAN
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