Enzymes Recombinant Proteins

Human CS Recombinant Protein (RPPB1507)

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RPPB1507

Description

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Product Name:Human CS Recombinant Protein
Product Code:RPPB1507
Size:20µg
Species:Human
Target:CS
Synonyms:Citrate Synthase, EC 2.3.3.1, Citrate (Si)-Synthase, EC2.3.3, Citrate synthase, mitochondrial.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:CS protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl,�10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSASSTNLK DILADLIPKE QARIKTFRQQ HGKTVVGQIT VDMMYGGMRGMKGLVYETSV LDPDEGIRFR GFSIPECQKL LPKAKGGEEP LPEGLFWLLV TGHIPTEEQV SWLSKEWAKRAALPSHVVTM LDNFPTNLHP MSQLSAAVTA LNSESNFARA YAQGISRTKY WELIYEDSMD LIAKLPCVAA KIYRNLYREG SGIGAIDSNLDWSHNFTNML GYTDHQFTEL TRLYLTIHSD HEGGNVSAHT SHLVGSALSD PYLSFAAAMN GLAGPLHGLANQEVLVWLTQ LQKEVGKDVS DEKLRDYIWN TLNSGRVVPG YGHAVLRKTD PRYTCQREFA LKHLPNDPMF KLVAQLYKIV PNVLLEQGKA KNPWPNVDAH SGVLLQYYGMTEMNYYTVLF GVSRALGVLA QLIWSRALGF PLERPKSMST EGLMKFVDSK SG

Citrate synthase also known as CS is a Krebs tricarboxylic acid cycleenzyme which catalyzes the synthesis of citrate from oxaloacetate and acetylcoenzyme A. CS is present in almost all cells capable of oxidative metabolism.CS is nuclear encoded and transported into the mitochondrial matrix, where themature form is found. The diseases related to CS are: critical illnesspolyneuropathy and mitochondrial cardiomyopathy.

CS Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 462 amino acids (28-466 a.a) andhaving a molecular mass of 51.4kDa.CS is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism.
UniProt Protein Details:

Protein type:EC 2.3.3.1; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Transferase

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function:citrate (Si)-synthase activity

Biological Process: cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; cellular carbohydrate metabolic process

NCBI Summary:The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008]
UniProt Code:O75390
NCBI GenInfo Identifier:57015285
NCBI Gene ID:1431
NCBI Accession:O75390.2
UniProt Secondary Accession:O75390,Q71UT9, Q7KZH0, Q96FZ8, Q9BWN8,
UniProt Related Accession:O75390
Molecular Weight:466
NCBI Full Name:Citrate synthase, mitochondrial
NCBI Synonym Full Names:citrate synthase
NCBI Official Symbol:CS��
NCBI Protein Information:citrate synthase, mitochondrial; citrate (Si)-synthase
UniProt Protein Name:Citrate synthase, mitochondrial
UniProt Synonym Protein Names:Citrate (Si)-synthase
Protein Family:Css54
UniProt Gene Name:CS��
UniProt Entry Name:CISY_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O75390
Research Area:
Enzymes
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