Human CRYAA Recombinant Protein (RPPB5193)

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RPPB5193

Description

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Product Name:Human CRYAA Recombinant Protein
Product Code:RPPB5193
Size:100µg
Species:Human
Target:CRYAA
Synonyms:CRYA1, HSPB4, CRYAA, Crystallin Alpha A, Alpha-crystallin A chain, Heat shock protein beta-4.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The CRYAA protein contains 20mM Tris-HCl buffer (pH 7.5), 50mM NaCl and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSGISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRLPSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein(sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of a-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.

Recombinant Human CRYAA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 19.9kDa.CRYAA is purified by proprietary chromatographic techniques.

UniProt Protein Function:CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens.
UniProt Protein Details:

Protein type:Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function:identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens

Biological Process: negative regulation of intracellular transport; visual perception; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis

Disease: Cataract 9, Multiple Types

NCBI Summary:Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
UniProt Code:P02489
NCBI GenInfo Identifier:1706112
NCBI Gene ID:1409
NCBI Accession:P02489.2
UniProt Secondary Accession:P02489,Q53X53,
UniProt Related Accession:P02489,AAB33370
Molecular Weight:173
NCBI Full Name:Alpha-crystallin A chain
NCBI Synonym Full Names:crystallin, alpha A
NCBI Official Symbol:CRYAA��
NCBI Official Synonym Symbols:CRYA1; HSPB4; CTRCT9��
NCBI Protein Information:alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1)
UniProt Protein Name:Alpha-crystallin A chain
UniProt Synonym Protein Names:Heat shock protein beta-4; HspB4
Protein Family:Alpha-crystallin
UniProt Gene Name:CRYAA��
UniProt Entry Name:CRYAA_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P02489
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