Human CRKL Recombinant Protein (RPPB3258)
- SKU:
- RPPB3258
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P46109
Description
| Product Name: | Human CRKL Recombinant Protein |
| Product Code: | RPPB3258 |
| Size: | 20µg |
| Species: | Human |
| Target: | CRKL |
| Synonyms: | Crk-like protein, CRKL. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The CRKL solution (1 mg/ml) 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRDSSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE |
Crk-like protein (CRKL) is a protein kinase containing SH2 and SH3 (src homology) domains which activates the RAS and JUN kinase signaling pathways and transforms fibroblasts in a RAS-dependent manner. CRKL is a substitute of the BCR-ABL tyrosine kinase, it also has a role in fibroblast transformation by BCR-ABL, and has oncogenic potential.
CRKL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 323 amino acids (1-303 a.a.) and having a molecular mass of 35.9kDa (Molecular size on SDS-PAGE will appear higher). The CRKL is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; endosome Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of MAPKK activity; JNK cascade; positive regulation of cell proliferation; Ras protein signal transduction |
| NCBI Summary: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009] |
| UniProt Code: | P46109 |
| NCBI GenInfo Identifier: | 1169094 |
| NCBI Gene ID: | 1399 |
| NCBI Accession: | P46109.1 |
| UniProt Secondary Accession: | P46109,A8KA44, D3DX35, |
| UniProt Related Accession: | P46109 |
| Molecular Weight: | 33,777 Da |
| NCBI Full Name: | Crk-like protein |
| NCBI Synonym Full Names: | CRK like proto-oncogene, adaptor protein |
| NCBI Official Symbol: | CRKL�� |
| NCBI Protein Information: | crk-like protein |
| UniProt Protein Name: | Crk-like protein |
| Protein Family: | Crk-like protein |
| UniProt Gene Name: | CRKL�� |
| UniProt Entry Name: | CRKL_HUMAN |
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